2ahj
From Proteopedia
Line 1: | Line 1: | ||
[[Image:2ahj.gif|left|200px]] | [[Image:2ahj.gif|left|200px]] | ||
- | + | <!-- | |
- | + | The line below this paragraph, containing "STRUCTURE_2ahj", creates the "Structure Box" on the page. | |
- | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
- | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
- | + | or leave the SCENE parameter empty for the default display. | |
- | | | + | --> |
- | | | + | {{STRUCTURE_2ahj| PDB=2ahj | SCENE= }} |
- | + | ||
- | + | ||
- | }} | + | |
'''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE''' | '''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE''' | ||
Line 35: | Line 32: | ||
[[Category: Tsujimura, M.]] | [[Category: Tsujimura, M.]] | ||
[[Category: Yohda, M.]] | [[Category: Yohda, M.]] | ||
- | [[Category: | + | [[Category: Hydratase]] |
- | [[Category: | + | [[Category: Lyase]] |
- | [[Category: | + | [[Category: Nitric oxide binding enzyme]] |
- | [[Category: | + | [[Category: Non-heme iron center]] |
- | [[Category: | + | [[Category: Photoreactive enzyme]] |
- | [[Category: | + | [[Category: Post-translational modification of cysteine residue]] |
- | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:03:26 2008'' | |
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + |
Revision as of 16:03, 3 May 2008
NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE
Overview
The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.
About this Structure
2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.
Reference
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994 Page seeded by OCA on Sat May 3 19:03:26 2008
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Endo, I. | Kamiya, N. | Nagashima, S. | Nakasako, M. | Odaka, M. | Takio, K. | Tsujimura, M. | Yohda, M. | Hydratase | Lyase | Nitric oxide binding enzyme | Non-heme iron center | Photoreactive enzyme | Post-translational modification of cysteine residue