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2alz

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[[Image:2alz.gif|left|200px]]
[[Image:2alz.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2alz |SIZE=350|CAPTION= <scene name='initialview01'>2alz</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2alz", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DCP:2&#39;-DEOXYCYTIDINE-5&#39;-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DOC:2&#39;,3&#39;-DIDEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= POLI, RAD30B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2alz| PDB=2alz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2alz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2alz OCA], [http://www.ebi.ac.uk/pdbsum/2alz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2alz RCSB]</span>
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}}
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'''Ternary Complex of hPoli with DNA and dCTP'''
'''Ternary Complex of hPoli with DNA and dCTP'''
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[[Category: Prakash, L.]]
[[Category: Prakash, L.]]
[[Category: Prakash, S.]]
[[Category: Prakash, S.]]
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[[Category: dna polymerase]]
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[[Category: Dna polymerase]]
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[[Category: hoogsteen base pair]]
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[[Category: Hoogsteen base pair]]
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[[Category: right handed]]
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[[Category: Right handed]]
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[[Category: template g. incoming dctp]]
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[[Category: Template g. incoming dctp]]
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[[Category: ternary complex]]
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[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:12:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:54:21 2008''
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Revision as of 16:12, 3 May 2008

Image:2alz.gif

Template:STRUCTURE 2alz

Ternary Complex of hPoli with DNA and dCTP


Overview

Human DNA polymerase iota (hPoliota), a member of the Y family of DNA polymerases, differs in remarkable ways from other DNA polymerases, incorporating correct nucleotides opposite template purines with a much higher efficiency and fidelity than opposite template pyrimidines. We present here the crystal structure of hPoliota bound to template G and incoming dCTP, which reveals a G.C + Hoogsteen base pair in a DNA polymerase active site. We show that the hPoliota active site has evolved to favor Hoogsteen base pairing, wherein the template sugar is fixed in a cavity that reduces the C1'-C1' distance across the nascent base pair from approximately 10.5 A in other DNA polymerases to 8.6 A in hPoliota. The rotation of G from anti to syn is then largely in response to this curtailed C1'-C1' distance. A G.C+ Hoogsteen base pair suggests a specific mechanism for hPoliota's ability to bypass N(2)-adducted guanines that obstruct replication.

About this Structure

2ALZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2005 Oct;13(10):1569-77. PMID:16216587 Page seeded by OCA on Sat May 3 19:12:16 2008

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