2aqx

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[[Image:2aqx.gif|left|200px]]
[[Image:2aqx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2aqx |SIZE=350|CAPTION= <scene name='initialview01'>2aqx</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2aqx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Inositol-trisphosphate_3-kinase Inositol-trisphosphate 3-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.127 2.7.1.127] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2aqx| PDB=2aqx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aqx OCA], [http://www.ebi.ac.uk/pdbsum/2aqx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aqx RCSB]</span>
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}}
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'''Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B'''
'''Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B'''
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[[Category: Sauer, K.]]
[[Category: Sauer, K.]]
[[Category: Spraggon, G.]]
[[Category: Spraggon, G.]]
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[[Category: calmodulin binding]]
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[[Category: Calmodulin binding]]
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[[Category: inositol]]
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[[Category: Inositol]]
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[[Category: ip3]]
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[[Category: Ip3]]
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[[Category: ip3-3k]]
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[[Category: Ip3-3k]]
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[[Category: ip3-3kb]]
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[[Category: Ip3-3kb]]
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[[Category: ip3k]]
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[[Category: Ip3k]]
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[[Category: itpkb]]
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[[Category: Itpkb]]
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[[Category: kinase]]
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[[Category: Kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:21:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:56:06 2008''
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Revision as of 16:21, 3 May 2008

Image:2aqx.gif

Template:STRUCTURE 2aqx

Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B


Overview

D-Myoinositol 1,4,5-trisphophate 3-kinases (IP(3)-3Ks) play important roles in metazoan cellular signaling. It has been demonstrated that mice without a functional version of IP(3)-3K isoform B are deficient in peripheral T-cells, indicating that IP(3)-3KB is essential to the developing immune system. The recent apo IP(3)-3KA structure exhibited a helix at the catalytic domain N-terminus exhibited a helix at the N-terminus of the catalytic domain, with a tryptophan indole moiety mimicking the binding mode of the substrate ATP purine ring, suggesting a mechanism of autoinhibition. Here we present the structure of the complete catalytic domain of IP(3)-3KB, including the CaM binding domain in complex with Mg(2+) and ATP. The crystal structure reveals a homodimeric arrangement of IP(3)-3KB catalytic domains, mediated via an intermolecular antiparallel beta-sheet formed from part of the CaM binding region. Residues from the putative autoinhibitory helix are rearranged into a loop configuration, with extensive interactions with the bound ATP. Mutagenesis of residues from this region reveals that substitution of the putative autoinhibitory tryptophan generates a hyperactive enzyme which retains Ca(2+)/CaM sensitivity. The IP(3)-3KB structure suggests a mechanism of enzyme activation, and raises the possibility that an interaction between IP(3)-3KB molecules may occur as part of the catalytic or regulatory cycle.

About this Structure

2AQX is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural insights into enzyme regulation for inositol 1,4,5-trisphosphate 3-kinase B., Chamberlain PP, Sandberg ML, Sauer K, Cooke MP, Lesley SA, Spraggon G, Biochemistry. 2005 Nov 8;44(44):14486-93. PMID:16262249 Page seeded by OCA on Sat May 3 19:21:50 2008

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