2aut

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[[Image:2aut.jpg|left|200px]]
[[Image:2aut.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2aut |SIZE=350|CAPTION= <scene name='initialview01'>2aut</scene>, resolution 2.25&Aring;
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The line below this paragraph, containing "STRUCTURE_2aut", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= aphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
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-->
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|DOMAIN=
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{{STRUCTURE_2aut| PDB=2aut | SCENE= }}
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|RELATEDENTRY=[[1z5g|1Z5G]], [[1z5u|1Z5U]], [[1z88|1Z88]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aut OCA], [http://www.ebi.ac.uk/pdbsum/2aut PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aut RCSB]</span>
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}}
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'''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''
'''Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium'''
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[[Category: Kumar, V.]]
[[Category: Kumar, V.]]
[[Category: Makde, R D.]]
[[Category: Makde, R D.]]
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[[Category: class-b bacterial non-specific acid phosphatase]]
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[[Category: Class-b bacterial non-specific acid phosphatase]]
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[[Category: lys154asn mutant of mature apha]]
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[[Category: Lys154asn mutant of mature apha]]
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[[Category: metalloenzyme]]
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[[Category: Metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:30:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:38 2008''
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Revision as of 16:30, 3 May 2008

Image:2aut.jpg

Template:STRUCTURE 2aut

Crystal structure of Lys154Asn mutant of mature AphA of S. typhimurium


Overview

The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.

About this Structure

2AUT is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135 Page seeded by OCA on Sat May 3 19:30:03 2008

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OCA

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