2ayn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2ayn.gif|left|200px]]
[[Image:2ayn.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2ayn |SIZE=350|CAPTION= <scene name='initialview01'>2ayn</scene>, resolution 3.2&Aring;
+
The line below this paragraph, containing "STRUCTURE_2ayn", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= USP14, TGT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2ayn| PDB=2ayn | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ayn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayn OCA], [http://www.ebi.ac.uk/pdbsum/2ayn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ayn RCSB]</span>
+
-
}}
+
'''Structure of USP14, a proteasome-associated deubiquitinating enzyme'''
'''Structure of USP14, a proteasome-associated deubiquitinating enzyme'''
Line 30: Line 27:
[[Category: Li, P.]]
[[Category: Li, P.]]
[[Category: Shi, Y.]]
[[Category: Shi, Y.]]
-
[[Category: deubiquitinating enzyme]]
+
[[Category: Deubiquitinating enzyme]]
-
[[Category: dub]]
+
[[Category: Dub]]
-
[[Category: enzyme mechanism]]
+
[[Category: Enzyme mechanism]]
-
[[Category: proteasome]]
+
[[Category: Proteasome]]
-
[[Category: usp14]]
+
[[Category: Usp14]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:37:55 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:59:05 2008''
+

Revision as of 16:37, 3 May 2008

Image:2ayn.gif

Template:STRUCTURE 2ayn

Structure of USP14, a proteasome-associated deubiquitinating enzyme


Overview

The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. Mammalian USP14 (Ubp6 in yeast) is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. Here, we report the crystal structures of the 45-kDa catalytic domain of USP14 in isolation and in a complex with ubiquitin aldehyde, which reveal distinct structural features. In the absence of ubiquitin binding, the catalytic cleft leading to the active site of USP14 is blocked by two surface loops. Binding by ubiquitin induces a significant conformational change that translocates the two surface loops thereby allowing access of the ubiquitin C-terminus to the active site. These structural observations, in conjunction with biochemical characterization, identify important regulatory mechanisms for USP14.

About this Structure

2AYN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14., Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y, EMBO J. 2005 Nov 2;24(21):3747-56. Epub 2005 Oct 6. PMID:16211010 Page seeded by OCA on Sat May 3 19:37:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ayn"
Personal tools