2b3q

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[[Image:2b3q.gif|left|200px]]
[[Image:2b3q.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2b3q |SIZE=350|CAPTION= <scene name='initialview01'>2b3q</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2b3q", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CRO:[2-(1-AMINO-2-HYDROXY-PROPYL)-4-(4-HYDROXY-BENZYLIDINE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETALDEHYDE'>CRO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= gft ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])
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|DOMAIN=
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{{STRUCTURE_2b3q| PDB=2b3q | SCENE= }}
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|RELATEDENTRY=[[1ema|1EMA]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3q OCA], [http://www.ebi.ac.uk/pdbsum/2b3q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b3q RCSB]</span>
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'''Crystal structure of a well-folded variant of green fluorescent protein'''
'''Crystal structure of a well-folded variant of green fluorescent protein'''
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[[Category: Waldo, G S.]]
[[Category: Waldo, G S.]]
[[Category: 11-stranded beta-barrel]]
[[Category: 11-stranded beta-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:48:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:03 2008''
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Revision as of 16:48, 3 May 2008

Image:2b3q.gif

Template:STRUCTURE 2b3q

Crystal structure of a well-folded variant of green fluorescent protein


Overview

Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

About this Structure

2B3Q is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Engineering and characterization of a superfolder green fluorescent protein., Pedelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS, Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. PMID:16369541 Page seeded by OCA on Sat May 3 19:48:56 2008

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