2b7v
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2b7v.gif|left|200px]] | [[Image:2b7v.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2b7v", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2b7v| PDB=2b7v | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Structure of ADAR2 dsRBM2''' | '''Structure of ADAR2 dsRBM2''' | ||
| Line 30: | Line 27: | ||
[[Category: Stefl, R.]] | [[Category: Stefl, R.]] | ||
[[Category: Xu, M.]] | [[Category: Xu, M.]] | ||
| - | [[Category: | + | [[Category: Rna editing]] |
| - | [[Category: | + | [[Category: Rna-binding protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:57:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:57, 3 May 2008
Structure of ADAR2 dsRBM2
Overview
Adenosine deaminases that act on RNA (ADARs) site-selectively modify adenosines to inosines within RNA transcripts, thereby recoding genomic information. How ADARs select specific adenosine moieties for deamination is poorly understood. Here, we report NMR structures of the two double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR chemical shift perturbation study of the interaction of the two dsRBMs with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes two bulged bases adjacent to the editing site, demonstrating RNA structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis studies with both the protein and the RNA further support our structural findings.
About this Structure
2B7V is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs., Stefl R, Xu M, Skrisovska L, Emeson RB, Allain FH, Structure. 2006 Feb;14(2):345-55. PMID:16472753 Page seeded by OCA on Sat May 3 19:57:52 2008
