2b8j
From Proteopedia
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'''Crystal structure of AphA class B acid phosphatase/phosphotransferase ternary complex with adenosine and phosphate at 2 A resolution''' | '''Crystal structure of AphA class B acid phosphatase/phosphotransferase ternary complex with adenosine and phosphate at 2 A resolution''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B8J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry | + | 2B8J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rm7 1rm7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B8J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rossolini, G M.]] | [[Category: Rossolini, G M.]] | ||
[[Category: Thaller, M C.]] | [[Category: Thaller, M C.]] | ||
| - | [[Category: | + | [[Category: Amp]] |
| - | [[Category: | + | [[Category: Class b acid phosphatase]] |
| - | [[Category: | + | [[Category: Dddd acid phosphatase]] |
| - | [[Category: | + | [[Category: Metallo-enzyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:59:00 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:59, 3 May 2008
Crystal structure of AphA class B acid phosphatase/phosphotransferase ternary complex with adenosine and phosphate at 2 A resolution
Overview
The Escherichia coli gene aphA codes for a periplasmic acid phosphatase called AphA, belonging to class B bacterial phosphatases, which is part of the DDDD superfamily of phosphohydrolases. After our first report about its crystal structure, we have started a series of crystallographic studies aimed at understanding of the catalytic mechanism of the enzyme. Here, we report three crystal structures of the AphA enzyme in complex with the hydrolysis products of nucleoside monophosphate substrates and a fourth with a proposed intermediate analogue that appears to be covalently bound to the enzyme. Comparison with the native enzyme structure and with the available X-ray structures of different phosphatases provides clues about the enzyme chemistry and allows us to propose a catalytic mechanism for AphA, and to discuss it with respect to the mechanism of other bacterial and human phosphatases.
About this Structure
2B8J is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1rm7. Full crystallographic information is available from OCA.
Reference
A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases., Calderone V, Forleo C, Benvenuti M, Thaller MC, Rossolini GM, Mangani S, J Mol Biol. 2006 Jan 27;355(4):708-21. Epub 2005 Nov 10. PMID:16330049 Page seeded by OCA on Sat May 3 19:59:00 2008
