2b8k

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[[Image:2b8k.gif|left|200px]]
[[Image:2b8k.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2b8k |SIZE=350|CAPTION= <scene name='initialview01'>2b8k</scene>, resolution 4.150&Aring;
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The line below this paragraph, containing "STRUCTURE_2b8k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2b8k| PDB=2b8k | SCENE= }}
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|RELATEDENTRY=[[1wcm|1WCM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b8k OCA], [http://www.ebi.ac.uk/pdbsum/2b8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b8k RCSB]</span>
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}}
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'''12-subunit RNA Polymerase II'''
'''12-subunit RNA Polymerase II'''
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[[Category: Ye, P.]]
[[Category: Ye, P.]]
[[Category: Zhang, M.]]
[[Category: Zhang, M.]]
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[[Category: cellular rna polymerase]]
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[[Category: Cellular rna polymerase]]
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[[Category: dna-dependent rna polymerase]]
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[[Category: Dna-dependent rna polymerase]]
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[[Category: dna-directed rna polymerase]]
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[[Category: Dna-directed rna polymerase]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: nuclear protein]]
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[[Category: Nuclear protein]]
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[[Category: transcription]]
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[[Category: Transcription]]
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[[Category: zinc]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:59:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:02:53 2008''
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Revision as of 16:59, 3 May 2008

Image:2b8k.gif

Template:STRUCTURE 2b8k

12-subunit RNA Polymerase II


Overview

Macromolecular assemblies as large as RNA polymerase II (Pol II) can be phased by a few intrinsically bound Zn atoms, by using MAD experiments as described here. A phasing effectiveness of 570 aa/Zn is attained for Pol II. The resulting experimental, unbiased electron density map is of such quality that it confirms the existing crystallographic model and further reveals structural regions not shown by model phases, thus updating the Pol II model at three sites. The mechanistically important fork loop-1 element is observed to be ordered in the absence of nucleic acids, suggesting additional insights into the mechanisms that maintain the stability of the transcription ternary complex and allow its release. Furthermore, a computational experiment with simulated MAD data sets demonstrates that 1 Zn site is able to provide adequate experimental phase information for as many as 1100 amino acids of polypeptide, under the conditions of the current synchrotron and detector technologies.

About this Structure

2B8K is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model., Meyer PA, Ye P, Zhang M, Suh MH, Fu J, Structure. 2006 Jun;14(6):973-82. PMID:16765890 Page seeded by OCA on Sat May 3 19:59:03 2008

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