2bam

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[[Image:2bam.gif|left|200px]]
[[Image:2bam.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2bam |SIZE=350|CAPTION= <scene name='initialview01'>2bam</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_2bam", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2bam| PDB=2bam | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bam OCA], [http://www.ebi.ac.uk/pdbsum/2bam PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bam RCSB]</span>
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}}
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'''RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND CALCIUM IONS (PRE-REACTIVE COMPLEX).'''
'''RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND CALCIUM IONS (PRE-REACTIVE COMPLEX).'''
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[[Category: Aggarwal, A K.]]
[[Category: Aggarwal, A K.]]
[[Category: Viadiu, H.]]
[[Category: Viadiu, H.]]
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[[Category: complex (endonuclease/dna)]]
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[[Category: Nuclease]]
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[[Category: nuclease]]
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[[Category: Phosphodiesterase]]
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[[Category: phosphodiesterase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:02:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:03:34 2008''
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Revision as of 17:02, 3 May 2008

Image:2bam.gif

Template:STRUCTURE 2bam

RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND CALCIUM IONS (PRE-REACTIVE COMPLEX).


Overview

Type II restriction enzymes are characterized by their remarkable specificity and simplicity. They require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. However, most of the structural work on endonucleases has been performed in the absence of metals, leaving unanswered questions about their mechanisms of DNA cleavage. Here we report structures of the endonuclease BamHI-DNA complex, determined in the presence of Mn2+ and Ca2+, that describe the enzyme at different stages of catalysis. Overall, the results support a two-metal mechanism of DNA cleavage for BamHI which is distinct from that of EcoRV.

About this Structure

2BAM is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.

Reference

The role of metals in catalysis by the restriction endonuclease BamHI., Viadiu H, Aggarwal AK, Nat Struct Biol. 1998 Oct;5(10):910-6. PMID:9783752 Page seeded by OCA on Sat May 3 20:02:45 2008

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