2bcx
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2bcx.jpg|left|200px]] | [[Image:2bcx.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2bcx", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2bcx| PDB=2bcx | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''Crystal structure of calmodulin in complex with a ryanodine receptor peptide''' | '''Crystal structure of calmodulin in complex with a ryanodine receptor peptide''' | ||
| Line 28: | Line 25: | ||
[[Category: Maximciuc, A A.]] | [[Category: Maximciuc, A A.]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
| - | [[Category: | + | [[Category: Ef-hand]] |
| - | [[Category: | + | [[Category: Type-2 turn]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:07:28 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:07, 3 May 2008
Crystal structure of calmodulin in complex with a ryanodine receptor peptide
Overview
Calmodulin regulates ryanodine receptor-mediated Ca(2+) release through a conserved binding site. The crystal structure of Ca(2+)-calmodulin bound to this conserved site reveals that calmodulin recognizes two hydrophobic anchor residues at a novel "1-17" spacing that brings the calmodulin lobes close together but prevents them from contacting one another. NMR residual dipolar couplings demonstrate that the detailed structure of each lobe is preserved in solution but also show that the lobes experience domain motions within the complex. FRET measurements confirm the close approach of the lobes in binding the 1-17 target and show that calmodulin binds with one lobe to a peptide lacking the second anchor. We suggest that calmodulin regulates the Ca(2+) channel by switching between the contiguous binding mode seen in our crystal structure and a state where one lobe of calmodulin contacts the conserved binding site while the other interacts with a noncontiguous site on the channel.
About this Structure
2BCX is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Complex of calmodulin with a ryanodine receptor target reveals a novel, flexible binding mode., Maximciuc AA, Putkey JA, Shamoo Y, Mackenzie KR, Structure. 2006 Oct;14(10):1547-56. PMID:17027503 Page seeded by OCA on Sat May 3 20:07:28 2008
