2be5

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[[Image:2be5.gif|left|200px]]
[[Image:2be5.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2be5 |SIZE=350|CAPTION= <scene name='initialview01'>2be5</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_2be5", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TGT:TAGETITOXIN'>TGT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2be5| PDB=2be5 | SCENE= }}
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|RELATEDENTRY=[[1iw7|1IW7]], [[1smy|1SMY]], [[2a6e|2A6E]], [[2a69|2A69]], [[2a68|2A68]], [[2a6h|2A6H]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2be5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2be5 OCA], [http://www.ebi.ac.uk/pdbsum/2be5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2be5 RCSB]</span>
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}}
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'''Crystal structure of the T. Thermophilus RNA polymerase holoenzyme in complex with inhibitor tagetitoxin'''
'''Crystal structure of the T. Thermophilus RNA polymerase holoenzyme in complex with inhibitor tagetitoxin'''
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[[Category: Vassylyeva, M N.]]
[[Category: Vassylyeva, M N.]]
[[Category: Wakatsuki, S.]]
[[Category: Wakatsuki, S.]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rna polymerase holoenzyme]]
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[[Category: Rna polymerase holoenzyme]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: tagetitoxin]]
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[[Category: Tagetitoxin]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:10:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:05:03 2008''
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Revision as of 17:10, 3 May 2008

Image:2be5.gif

Template:STRUCTURE 2be5

Crystal structure of the T. Thermophilus RNA polymerase holoenzyme in complex with inhibitor tagetitoxin


Overview

Tagetitoxin (Tgt) inhibits transcription by an unknown mechanism. A structure at a resolution of 2.4 A of the Thermus thermophilus RNA polymerase (RNAP)-Tgt complex revealed that the Tgt-binding site within the RNAP secondary channel overlaps that of the stringent control effector ppGpp, which partially protects RNAP from Tgt inhibition. Tgt binding is mediated exclusively through polar interactions with the beta and beta' residues whose substitutions confer resistance to Tgt in vitro. Importantly, a Tgt phosphate, together with two active site acidic residues, coordinates the third Mg(2+) ion, which is distinct from the two catalytic metal ions. We show that Tgt inhibits all RNAP catalytic reactions and propose a mechanism in which the Tgt-bound Mg(2+) ion has a key role in stabilization of an inactive transcription intermediate. Remodeling of the active site by metal ions could be a common theme in the regulation of catalysis by nucleic acid enzymes.

About this Structure

2BE5 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural basis for transcription inhibition by tagetitoxin., Vassylyev DG, Svetlov V, Vassylyeva MN, Perederina A, Igarashi N, Matsugaki N, Wakatsuki S, Artsimovitch I, Nat Struct Mol Biol. 2005 Dec;12(12):1086-93. Epub 2005 Nov 6. PMID:16273103 Page seeded by OCA on Sat May 3 20:10:00 2008

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