2bfn

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[[Image:2bfn.gif|left|200px]]
[[Image:2bfn.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2bfn |SIZE=350|CAPTION= <scene name='initialview01'>2bfn</scene>, resolution 1.600&Aring;
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The line below this paragraph, containing "STRUCTURE_2bfn", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:D2p+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cl+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Ca+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Ca+Binding+Site+For+Chain+A'>AC5</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D2P:(2S)-2,3-DICHLOROPROPAN-1-OL'>D2P</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2bfn| PDB=2bfn | SCENE= }}
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|RELATEDENTRY=[[1cv2|1CV2]], [[1d07|1D07]], [[1g42|1G42]], [[1g4h|1G4H]], [[1g5f|1G5F]], [[1iz7|1IZ7]], [[1iz8|1IZ8]], [[1k5p|1K5P]], [[1k63|1K63]], [[1k6e|1K6E]], [[1mj5|1MJ5]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfn OCA], [http://www.ebi.ac.uk/pdbsum/2bfn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bfn RCSB]</span>
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}}
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'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''
'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''
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==About this Structure==
==About this Structure==
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2BFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFN OCA].
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2BFN is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFN OCA].
==Reference==
==Reference==
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[[Category: Vevodova, J.]]
[[Category: Vevodova, J.]]
[[Category: 1,2,3-trichloropropane]]
[[Category: 1,2,3-trichloropropane]]
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[[Category: alpha/beta-hydrolase]]
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[[Category: Alpha/beta-hydrolase]]
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[[Category: haloalkane dehalogenase linb]]
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[[Category: Haloalkane dehalogenase linb]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:13:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:05:41 2008''
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Revision as of 17:13, 3 May 2008

Image:2bfn.gif

Template:STRUCTURE 2bfn

THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.


Overview

1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities.

About this Structure

2BFN is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:17259360 Page seeded by OCA on Sat May 3 20:13:29 2008

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