2bgy
From Proteopedia
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'''FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.''' | '''FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.''' | ||
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[[Category: Shaikh, T R.]] | [[Category: Shaikh, T R.]] | ||
[[Category: Thomas, D R.]] | [[Category: Thomas, D R.]] | ||
| - | [[Category: | + | [[Category: Bacterial motility]] |
| - | [[Category: | + | [[Category: Electron microscopy]] |
| - | [[Category: | + | [[Category: Flagellar hook]] |
| - | [[Category: | + | [[Category: Flagellum]] |
| - | [[Category: | + | [[Category: Model building]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:16, 3 May 2008
FIT OF THE X-RAY STRUCUTRE OF THE BATERIAL FLAGELLAR HOOK FRAGMENT FLGE31 INTO AN EM MAP FROM THE HOOK OF CAULOBACTER CRESCENTUS.
Overview
The axial proteins of the bacterial flagellum function as a drive shaft, universal joint, and propeller driven by the flagellar rotary motor; they also form the putative protein export channel. The N- and C-terminal sequences of the eight axial proteins were predicted to form interlocking alpha-domains generating an axial tube. We report on an approximately 1-nm resolution map of the hook from Salmonella typhimurium, which reveals such a tube made from interdigitated, 1-nm rod-like densities similar to those seen in maps of the filament. Atomic models for the two outer domains of the hook subunit were docked into the corresponding outermost features of the map. The N and C termini of the hook subunit fragment are positioned next to each other and face toward the axis of the hook. The placement of these termini would permit the residues missing in the fragment to form the rod-like features that form the core domain of the hook. We also fit the hook atomic model to an approximately 2-nm resolution map of the hook from Caulobacter crescentus. The hook protein sequence from C. crescentus is largely homologous to that of S. typhimurium except for a large insertion (20 kDa). According to difference maps and our fitting, this insertion is found on the outer surface of the hook, consistent with our modeling of the hook.
About this Structure
2BGY is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
A partial atomic structure for the flagellar hook of Salmonella typhimurium., Shaikh TR, Thomas DR, Chen JZ, Samatey FA, Matsunami H, Imada K, Namba K, Derosier DJ, Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1023-8. Epub 2005 Jan 18. PMID:15657146 Page seeded by OCA on Sat May 3 20:16:20 2008
