2bir

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[[Image:2bir.jpg|left|200px]]
[[Image:2bir.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2bir |SIZE=350|CAPTION= <scene name='initialview01'>2bir</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2bir", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=S1:Catalytic+Site'>S1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=2GP:GUANOSINE-2&#39;-MONOPHOSPHATE'>2GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2bir| PDB=2bir | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bir OCA], [http://www.ebi.ac.uk/pdbsum/2bir PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bir RCSB]</span>
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}}
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'''ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)'''
'''ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)'''
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[[Category: Loverix, S.]]
[[Category: Loverix, S.]]
[[Category: Steyaert, J.]]
[[Category: Steyaert, J.]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: nuclease]]
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[[Category: Nuclease]]
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[[Category: ribonuclease]]
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[[Category: Ribonuclease]]
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[[Category: signal]]
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[[Category: Signal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:20:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:03 2008''
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Revision as of 17:20, 3 May 2008

Image:2bir.jpg

Template:STRUCTURE 2bir

ADDITIVITY OF SUBSTRATE BINDING IN RIBONUCLEASE T1 (Y42A MUTANT)


Overview

It has been established that Tyr-42, Tyr-45, and Glu-46 take part in a structural motif that renders guanine specificity to ribonuclease T1. We report on the impact of Tyr-42, Tyr-45, and Glu-46 substitutions on the guanine specificity of RNase T1. The Y42A and E46A mutations profoundly affect substrate binding. No such effect is observed for Y45A RNase T1. From the kinetics of the Y42A/Y45A and Y42A/E46A double mutants, we conclude that these pairs of residues contribute to guanine specificity in a mutually independent way. From our results, it appears that the energetic contribution of aromatic face-to-face stacking interactions may be significant if polycyclic molecules, such as guanine, are involved.

About this Structure

2BIR is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Additivity of protein-guanine interactions in ribonuclease T1., Loverix S, Doumen J, Steyaert J, J Biol Chem. 1997 Apr 11;272(15):9635-9. PMID:9092491 Page seeded by OCA on Sat May 3 20:20:50 2008

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