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2bjv

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[[Image:2bjv.gif|left|200px]]
[[Image:2bjv.gif|left|200px]]
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{{Structure
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{{STRUCTURE_2bjv| PDB=2bjv | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjv OCA], [http://www.ebi.ac.uk/pdbsum/2bjv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bjv RCSB]</span>
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'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''
'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''
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[[Category: Wigneshweraraj, S.]]
[[Category: Wigneshweraraj, S.]]
[[Category: Zhang, X.]]
[[Category: Zhang, X.]]
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[[Category: aaa,transcription activation]]
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[[Category: Aaa,transcription activation]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: enhancer binding protein]]
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[[Category: Enhancer binding protein]]
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[[Category: gene regulation]]
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[[Category: Gene regulation]]
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[[Category: pspf]]
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[[Category: Pspf]]
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[[Category: sigma54 activator]]
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[[Category: Sigma54 activator]]
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[[Category: transcription regulation,]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:23:16 2008''
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Revision as of 17:23, 3 May 2008

Image:2bjv.gif

Template:STRUCTURE 2bjv

CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT


Overview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this Structure

2BJV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859 Page seeded by OCA on Sat May 3 20:23:16 2008

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