2bk9

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[[Image:2bk9.gif|left|200px]]
[[Image:2bk9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2bk9 |SIZE=350|CAPTION= <scene name='initialview01'>2bk9</scene>, resolution 1.20&Aring;
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The line below this paragraph, containing "STRUCTURE_2bk9", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Hem+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Cxs+Binding+Site+For+Chain+A'>AC2</scene> and <scene name='pdbsite=AC3:Cl+Binding+Site+For+Chain+A'>AC3</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2bk9| PDB=2bk9 | SCENE= }}
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|RELATEDENTRY=[[2g3h|2G3H]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bk9 OCA], [http://www.ebi.ac.uk/pdbsum/2bk9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bk9 RCSB]</span>
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}}
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'''DROSOPHILA MELANOGASTER GLOBIN'''
'''DROSOPHILA MELANOGASTER GLOBIN'''
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[[Category: Ponassi, M.]]
[[Category: Ponassi, M.]]
[[Category: Sanctis, D De.]]
[[Category: Sanctis, D De.]]
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[[Category: drosophila melanogaster hemoglobin]]
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[[Category: Drosophila melanogaster hemoglobin]]
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[[Category: heme hexacoordination]]
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[[Category: Heme hexacoordination]]
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[[Category: insect hemoglobin]]
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[[Category: Insect hemoglobin]]
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[[Category: protein cavity]]
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[[Category: Protein cavity]]
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[[Category: protein structure oxygen transport]]
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[[Category: Protein structure oxygen transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:24:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:37 2008''
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Revision as of 17:24, 3 May 2008

Image:2bk9.gif

Template:STRUCTURE 2bk9

DROSOPHILA MELANOGASTER GLOBIN


Overview

Hemoglobins at high concentration have been isolated long ago from some insect larvae living in hypoxic environments. Conversely, a monomeric hemoglobin has been discovered recently in the fruit fly Drosophila melanogaster as intracellular protein expressed both in larvae and in the adult fly. Such a finding indicates that the oxygen supply in insects may be more complex than previously thought, relying not only on O2 diffusion through the tubular tracheal system, but also on carrier-mediated transport and storage. We present here the crystal structure of recombinant D. melanogaster hemoglobin at 1.20 A resolution. Spectroscopic data show that the protein displays a hexacoordinated heme, whose axial ligands are the proximal and distal His residues. Such bis-His ligation of the heme has sizable effects on the protein local structure. Three protein matrix cavities, comparable in size but not in topological locations with those of sperm whale myoglobin, are spread through the protein matrix; one of these can host a xenon atom. Additionally, D. melanogaster hemoglobin binds one molecule of 3-(cyclohexylamino)propanesulfonic acid (CAPS) buffer at a surface pocket, next to the EF hinge. Despite the high resolution achieved, no sequence/structure features specifically supporting the heme hexa- to pentacoordination transition required for diatomic ligand binding could be recognized.

About this Structure

2BK9 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Bishistidyl heme hexacoordination, a key structural property in Drosophila melanogaster hemoglobin., de Sanctis D, Dewilde S, Vonrhein C, Pesce A, Moens L, Ascenzi P, Hankeln T, Burmester T, Ponassi M, Nardini M, Bolognesi M, J Biol Chem. 2005 Jul 22;280(29):27222-9. Epub 2005 May 24. PMID:15917230 Page seeded by OCA on Sat May 3 20:24:15 2008

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OCA

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