2bkj

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[[Image:2bkj.jpg|left|200px]]
[[Image:2bkj.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2bkj |SIZE=350|CAPTION= <scene name='initialview01'>2bkj</scene>, resolution 2.08&Aring;
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The line below this paragraph, containing "STRUCTURE_2bkj", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2bkj| PDB=2bkj | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkj OCA], [http://www.ebi.ac.uk/pdbsum/2bkj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bkj RCSB]</span>
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}}
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'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+'''
'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+'''
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[[Category: TU, S C.]]
[[Category: TU, S C.]]
[[Category: Tanner, J J.]]
[[Category: Tanner, J J.]]
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[[Category: frp]]
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[[Category: Frp]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:25:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:45 2008''
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Revision as of 17:25, 3 May 2008

Image:2bkj.jpg

Template:STRUCTURE 2bkj

NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+


Overview

The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from the extended conformations observed in other enzyme/NAD(P) structures; however, it resembles the conformation proposed for NAD in solution. The flavin reductase P/NAD structure provides new information about the conformational diversity of NAD, which is important for understanding catalysis. This structure offers the first crystallographic evidence of a folded NAD with ring stacking, and it is the first enzyme structure containing an FMN cofactor interacting with NAD(P). Analysis of the structure suggests a possible dynamic mechanism underlying NADPH substrate specificity and product release that involves unfolding and folding of NADP(H).

About this Structure

2BKJ is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.

Reference

Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P., Tanner JJ, Tu SC, Barbour LJ, Barnes CL, Krause KL, Protein Sci. 1999 Sep;8(9):1725-32. PMID:10493573 Page seeded by OCA on Sat May 3 20:25:00 2008

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OCA

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