2bkm

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[[Image:2bkm.jpg|left|200px]]
[[Image:2bkm.jpg|left|200px]]
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{{Structure
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|PDB= 2bkm |SIZE=350|CAPTION= <scene name='initialview01'>2bkm</scene>, resolution 1.50&Aring;
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The line below this paragraph, containing "STRUCTURE_2bkm", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+L'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>
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{{STRUCTURE_2bkm| PDB=2bkm | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bkm OCA], [http://www.ebi.ac.uk/pdbsum/2bkm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bkm RCSB]</span>
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'''CRYSTAL STRUCTURE OF THE TRUNCATED HEMOGLOBIN FROM GEOBACILLUS STEAROTHERMOPHILUS'''
'''CRYSTAL STRUCTURE OF THE TRUNCATED HEMOGLOBIN FROM GEOBACILLUS STEAROTHERMOPHILUS'''
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[[Category: Kjelgaard, P.]]
[[Category: Kjelgaard, P.]]
[[Category: Wachenfeldt, C Von.]]
[[Category: Wachenfeldt, C Von.]]
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[[Category: hypothetical protein]]
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[[Category: Hypothetical protein]]
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[[Category: oxygen storage]]
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[[Category: Oxygen storage]]
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[[Category: oxygen transport]]
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[[Category: Oxygen transport]]
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[[Category: transport]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:25:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:07:50 2008''
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Revision as of 17:25, 3 May 2008

Image:2bkm.jpg

Template:STRUCTURE 2bkm

CRYSTAL STRUCTURE OF THE TRUNCATED HEMOGLOBIN FROM GEOBACILLUS STEAROTHERMOPHILUS


Overview

A novel truncated hemoglobin has been identified in the thermophilic bacterium Geobacillus stearothermophilus (Gs-trHb). The protein has been expressed in Escherichia coli, the 3D crystal structure (at 1.5 Angstroms resolution) and the ligand binding properties have been determined. The distal heme pocket displays an array of hydrogen bonding donors to the iron-bound ligands, including Tyr-B10 on one side of the heme pocket and Trp-G8 indole nitrogen on the opposite side. At variance with the highly similar Bacillus subtilis hemoglobin, Gs-trHb is dimeric both in the crystal and in solution and displays several unique structural properties. In the crystal cell, the iron-bound ligand is not homogeneously distributed within each distal site such that oxygen and an acetate anion can be resolved with relative occupancies of 50% each. Accordingly, equilibrium titrations of the oxygenated derivative in solution with acetate anion yield a partially saturated ferric acetate adduct. Moreover, the asymmetric unit contains two subunits and sedimentation velocity ultracentrifugation data confirm that the protein is dimeric.

About this Structure

2BKM is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure and ligand binding properties of the truncated hemoglobin from Geobacillus stearothermophilus., Ilari A, Kjelgaard P, von Wachenfeldt C, Catacchio B, Chiancone E, Boffi A, Arch Biochem Biophys. 2007 Jan 1;457(1):85-94. Epub 2006 Oct 30. PMID:17126283 Page seeded by OCA on Sat May 3 20:25:18 2008

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