2bl6
From Proteopedia
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| - | + | {{STRUCTURE_2bl6| PDB=2bl6 | SCENE= }} | |
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'''SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.''' | '''SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BL6 is a [[Single protein]] structure | + | 2BL6 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Castiglione-Morelli, M A.]] | [[Category: Castiglione-Morelli, M A.]] | ||
[[Category: Ostuni, A.]] | [[Category: Ostuni, A.]] | ||
| - | [[Category: | + | [[Category: Core protein]] |
| - | [[Category: | + | [[Category: Lentivirus]] |
| - | [[Category: | + | [[Category: Nucleocapsid protein]] |
| - | [[Category: | + | [[Category: Polyprotein]] |
| - | [[Category: | + | [[Category: Retrovirus zinc finger-like domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:26:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:26, 3 May 2008
SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.
Overview
Lentiviral nucleocapsid proteins are a class of multifunctional proteins that play an essential role in RNA packaging and viral infectivity. They contain two CX(2)CX(4)HX(4)C zinc binding motifs connected by a basic linker of variable length. The 3D structure of a 37-aa peptide corresponding to sequence 22-58 from lentiviral EIAV nucleocapsid protein NCp11, complexed with zinc, has been determined by 2D (1)H NMR spectroscopy, simulated annealing, and molecular dynamics. The solution structure consists of two zinc binding domains held together by a five-residue basic linker Arg(38)-Ala-Pro-Lys-Val(42) that allows for spatial proximity between the two finger domains. Observed linker folding is stabilized by H bonded secondary structure elements, resulting in an Omega-shaped central region, asymmetrically centered on the linker. The conformational differences and similarities with other NC zinc binding knuckles have been systematically analyzed. The two CCHC motifs, both characterized by a peculiar Pro-Gly sequence preceding the His residue, although preserving Zn-binding geometry and chirality of other known NC proteins, exhibit local fold differences both between each other and in comparison with other previously characterized retroviral CCHC motifs.
About this Structure
2BL6 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structural features in EIAV NCp11: a lentivirus nucleocapsid protein with a short linker., Amodeo P, Castiglione Morelli MA, Ostuni A, Battistuzzi G, Bavoso A, Biochemistry. 2006 May 2;45(17):5517-26. PMID:16634633 Page seeded by OCA on Sat May 3 20:26:32 2008
