2f9j
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(New page: 200px<br /> <applet load="2f9j" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f9j, resolution 3.00Å" /> '''3.0 angstrom resolu...)
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Revision as of 19:55, 12 November 2007
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3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155
Overview
The precise excision of introns from precursor mRNAs (pre-mRNAs) in, eukaryotes is accomplished by the spliceosome, a complex assembly, containing five small nuclear ribonucleoprotein (snRNP) particles. Human, p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown, to associate directly with the pre-mRNA branch adenosine early in, spliceosome assembly and within the fully assembled spliceosome. Here we, report the 2.5-A crystal structure of a complex containing p14 and a, peptide derived from the p14-associated U2 snRNP component SF3b155. p14, contains an RNA recognition motif (RRM), the surface of which is largely, occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide., An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows, that the branch adenosine directly interacts with a conserved aromatic, within a pocket on the surface of the complex. This result, combined with, a comparison of the structure with known RRMs and pseudoRRMs as well as, model-building by using the electron cryomicroscopy structure of a, spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a, noncanonical surface for RNA recognition at the heart of the mammalian, spliceosome.
About this Structure
2F9J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a core spliceosomal protein interface., Schellenberg MJ, Edwards RA, Ritchie DB, Kent OA, Golas MM, Stark H, Luhrmann R, Glover JN, MacMillan AM, Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1266-71. Epub 2006 Jan 23. PMID:16432215
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