2fdp

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Revision as of 19:57, 12 November 2007

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spinqualitylabelsall models 2fdp, resolution 2.500Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of beta-secretase complexed with an amino-ethylene inhibitor

Overview

A series of novel beta-site amyloid precursor protein cleaving enzyme, (BACE-1) inhibitors containing an aminoethylene (AE) tetrahedral, intermediate isostere were synthesized and evaluated in comparison to, corresponding hydroxyethylene (HE) compounds. Enzymatic inhibitory values, were similar for both isosteres, as were structure-activity relationships, with respect to stereochemical preference and substituent variation, (P2/P3, P1, and P2'); however, the AE compounds were markedly more potent, in a cell-based assay for reduction of beta-secretase activity. The, incorporation of preferred P2/P3, P1, and P2' substituents into the AE, pharmacophore yielded compound 7, which possessed enzymatic and cell assay, IC(50)s of 26 nM and 180 nM, respectively. A three-dimensional crystal, structure of 7 in complex with BACE-1 revealed that the amino group of the, inhibitor core engages the catalytic aspartates in a manner analogous to, hydroxyl groups in HE inhibitors. The AE isostere class represents a, promising advance in the development of BACE-1 inhibitors.

About this Structure

2FDP is a Single protein structure of sequence from Homo sapiens with FRP as ligand. Active as Memapsin 2, with EC number 3.4.23.46 Full crystallographic information is available from OCA.

Reference

Aminoethylenes: a tetrahedral intermediate isostere yielding potent inhibitors of the aspartyl protease BACE-1., Yang W, Lu W, Lu Y, Zhong M, Sun J, Thomas AE, Wilkinson JM, Fucini RV, Lam M, Randal M, Shi XP, Jacobs JW, McDowell RS, Gordon EM, Ballinger MD, J Med Chem. 2006 Feb 9;49(3):839-42. PMID:16451048

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