2c13

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[[Image:2c13.gif|left|200px]]
[[Image:2c13.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2c13 |SIZE=350|CAPTION= <scene name='initialview01'>2c13</scene>, resolution 2.150&Aring;
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The line below this paragraph, containing "STRUCTURE_2c13", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:K+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FHL:(E)-N~6~-[3-CARBOXY-1-(HYDROXYMETHYL)PROPYLIDENE]-L-LYSINE'>FHL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2c13| PDB=2c13 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c13 OCA], [http://www.ebi.ac.uk/pdbsum/2c13 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c13 RCSB]</span>
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}}
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'''5-HYDROXY-LEVULINIC ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA'''
'''5-HYDROXY-LEVULINIC ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA'''
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[[Category: Neier, R.]]
[[Category: Neier, R.]]
[[Category: Nentwich, M.]]
[[Category: Nentwich, M.]]
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[[Category: cocrystallization]]
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[[Category: Cocrystallization]]
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[[Category: enzyme mechanism]]
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[[Category: Enzyme mechanism]]
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[[Category: lyase]]
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[[Category: Lyase]]
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[[Category: metalloenzyme]]
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[[Category: Metalloenzyme]]
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[[Category: porphobilinogen synthase]]
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[[Category: Porphobilinogen synthase]]
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[[Category: porphyrin biosynthesis]]
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[[Category: Porphyrin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:05:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:14:45 2008''
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Revision as of 18:05, 3 May 2008

Image:2c13.gif

Template:STRUCTURE 2c13

5-HYDROXY-LEVULINIC ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA


Overview

Porphobilinogen synthase catalyzes the first committed step of the tetrapyrrole biosynthesis pathway. In an aldol-like condensation, two molecules of 5-aminolevulinic acid (ALA) form the first pyrrole, porphobilinogen. Newly synthesized analogues of a reaction intermediate of porphobilinogen synthase have been employed in studying the active site and the catalytic mechanism of this early enzyme of tetrapyrrole biosynthesis. This study combines structural and kinetic evaluation of the inhibition potency of these inhibitors. In addition, one of the determined protein structures provides for the first time structural evidence of a magnesium ion in the active site. From these results, we can corroborate an earlier postulated enzymatic mechanism that starts with formation of a C-C bond, linking C3 of the A-side ALA to C4 of the P-side ALA through an aldole addition. The obtained data are discussed with respect to the current literature.

About this Structure

2C13 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors., Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N, Biochemistry. 2006 Jul 11;45(27):8243-53. PMID:16819823 Page seeded by OCA on Sat May 3 21:05:08 2008

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