2c3y

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[[Image:2c3y.jpg|left|200px]]
[[Image:2c3y.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2c3y |SIZE=350|CAPTION= <scene name='initialview01'>2c3y</scene>, resolution 1.93&Aring;
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The line below this paragraph, containing "STRUCTURE_2c3y", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Co2+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=HTL:2-ACETYL-THIAMINE+DIPHOSPHATE'>HTL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_synthase Pyruvate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.7.1 1.2.7.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2c3y| PDB=2c3y | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c3y OCA], [http://www.ebi.ac.uk/pdbsum/2c3y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c3y RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS'''
'''CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS'''
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[[Category: Pieulle, L.]]
[[Category: Pieulle, L.]]
[[Category: 4fe-4]]
[[Category: 4fe-4]]
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[[Category: electron transport]]
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[[Category: Electron transport]]
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[[Category: iron]]
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[[Category: Iron]]
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[[Category: iron-sulfur]]
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[[Category: Iron-sulfur]]
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[[Category: iron-sulfur cluster]]
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[[Category: Iron-sulfur cluster]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: pyruvate catabolism]]
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[[Category: Pyruvate catabolism]]
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[[Category: tpp-dependent enzyme]]
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[[Category: Tpp-dependent enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:12:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:15:59 2008''
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Revision as of 18:12, 3 May 2008

Image:2c3y.jpg

Template:STRUCTURE 2c3y

CRYSTAL STRUCTURE OF THE RADICAL FORM OF PYRUVATE:FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS


Overview

Pyruvate-ferredoxin oxidoreductases (PFOR) are unique among thiamine pyrophosphate (ThDP)-containing enzymes in giving rise to a rather stable cofactor-based free-radical species upon the decarboxylation of their first substrate, pyruvate. We have obtained snapshots of unreacted and partially reacted (probably as a tetrahedral intermediate) pyruvate-PFOR complexes at different time intervals. We conclude that pyruvate decarboxylation involves very limited substrate-to-product movements but a significant displacement of the thiazolium moiety of ThDP. In this respect, PFOR seems to differ substantially from other ThDP-containing enzymes, such as transketolase and pyruvate decarboxylase. In addition, exposure of PFOR to oxygen in the presence of pyruvate results in significant inhibition of catalytic activity, both in solution and in the crystals. Examination of the crystal structure of inhibited PFOR suggests that the loss of activity results from oxime formation at the 4' amino substituent of the pyrimidine moiety of ThDP.

About this Structure

2C3Y is a Single protein structure of sequence from Desulfovibrio africanus. Full crystallographic information is available from OCA.

Reference

Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate., Cavazza C, Contreras-Martel C, Pieulle L, Chabriere E, Hatchikian EC, Fontecilla-Camps JC, Structure. 2006 Feb;14(2):217-24. PMID:16472741 Page seeded by OCA on Sat May 3 21:12:46 2008

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