2c45
From Proteopedia
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[[Image:2c45.jpg|left|200px]] | [[Image:2c45.jpg|left|200px]] | ||
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| - | | | + | {{STRUCTURE_2c45| PDB=2c45 | SCENE= }} |
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'''NATIVE PRECURSOR OF PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE''' | '''NATIVE PRECURSOR OF PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C45 is a [[Single protein]] structure | + | 2C45 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C45 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Ranganathan, A.]] | [[Category: Ranganathan, A.]] | ||
[[Category: Swaminathan, K.]] | [[Category: Swaminathan, K.]] | ||
| - | [[Category: | + | [[Category: Carboxylase]] |
| - | [[Category: | + | [[Category: Decarboxylase]] |
| - | [[Category: | + | [[Category: Double-psi beta barrel]] |
| - | [[Category: | + | [[Category: Lyase]] |
| - | [[Category: | + | [[Category: Pantothenate biosynthesis]] |
| - | [[Category: | + | [[Category: Pyruvate]] |
| - | [[Category: | + | [[Category: Zymogen]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:13:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:13, 3 May 2008
NATIVE PRECURSOR OF PYRUVOYL DEPENDENT ASPARTATE DECARBOXYLASE
Overview
L-aspartate-alpha-decarboxylase (ADC) is a critical regulatory enzyme in the pantothenate biosynthetic pathway and belongs to a small class of self-cleaving and pyruvoyl-dependent amino acid decarboxylases. The expression level of ADC in Mycobacterium tuberculosis (Mtb) was confirmed by cDNA analysis, immunoblotting with an anti-ADC polyclonal antibody using whole cell lysate and immunoelectron microscopy. The recombinant ADC proenzyme from Mycobacterium tuberculosis (MtbADC) was overexpressed in E. coli and the protein structure was determined at 2.99 A resolution. The proteins fold into the double-psi beta-barrel structure. The subunits of the two tetramers (there are eight ADC molecules in the asymmetric unit) form pseudo fourfold rotational symmetry, similar to the E. coli ADC proenzyme structure. As pantothenate is synthesized in microorganisms, plants, and fungi but not in animals, structure elucidation of Mtb ADC is of substantial interest for structure-based drug development.
About this Structure
2C45 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Crystal structure of uncleaved L-aspartate-alpha-decarboxylase from Mycobacterium tuberculosis., Gopalan G, Chopra S, Ranganathan A, Swaminathan K, Proteins. 2006 Dec 1;65(4):796-802. PMID:17001646 Page seeded by OCA on Sat May 3 21:13:22 2008
