2c9k
From Proteopedia
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'''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION''' | '''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION''' | ||
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[[Category: Lescar, J.]] | [[Category: Lescar, J.]] | ||
[[Category: Mo, M.]] | [[Category: Mo, M.]] | ||
| - | [[Category: | + | [[Category: Bioinsecticicide]] |
| - | [[Category: | + | [[Category: Insect toxin]] |
| - | [[Category: | + | [[Category: Pore formation]] |
| - | [[Category: | + | [[Category: Receptor recognition]] |
| - | [[Category: | + | [[Category: Sporulation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:30:58 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:30, 3 May 2008
STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION
Overview
The Cry4Aa delta-endotoxin from Bacillus thuringiensis is toxic to larvae of Culex, Anopheles, and Aedes mosquitoes, which are vectors of important human tropical diseases. With the objective of designing modified toxins with improved potency that could be used as biopesticides, we determined the structure of this toxin in its functional form at a resolution of 2.8 angstroms. Like other Cry delta-endotoxins, the activated Cry4Aa toxin consists of three globular domains, a seven-alpha-helix bundle responsible for pore formation (domain I) and the following two other domains having structural similarities with carbohydrate binding proteins: a beta-prism (domain II) and a plant lectin-like beta-sandwich (domain III). We also studied the effect on toxicity of amino acid substitutions and deletions in three loops located at the surface of the putative receptor binding domain II of Cry4Aa. Our results indicate that one loop is an important determinant of toxicity, presumably through attachment of Cry4Aa to the surface of mosquito cells. The availability of the Cry4Aa structure should guide further investigations aimed at the molecular basis of the target specificity and membrane insertion of Cry endotoxins.
About this Structure
2C9K is a Single protein structure of sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA.
Reference
Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution., Boonserm P, Mo M, Angsuthanasombat C, Lescar J, J Bacteriol. 2006 May;188(9):3391-401. PMID:16621834 Page seeded by OCA on Sat May 3 21:30:58 2008
