2cay
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2cay.gif|left|200px]] | [[Image:2cay.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2cay", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | | | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | + | --> | |
| - | + | {{STRUCTURE_2cay| PDB=2cay | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''VPS36 N-TERMINAL PH DOMAIN''' | '''VPS36 N-TERMINAL PH DOMAIN''' | ||
| Line 29: | Line 26: | ||
[[Category: Teo, H.]] | [[Category: Teo, H.]] | ||
[[Category: Williams, R L.]] | [[Category: Williams, R L.]] | ||
| - | [[Category: | + | [[Category: Escrt-ii]] |
| - | [[Category: | + | [[Category: Lipid-binding]] |
| - | [[Category: | + | [[Category: Membrane]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Multivesicular body]] |
| - | [[Category: | + | [[Category: Ph domain]] |
| - | [[Category: | + | [[Category: Protein transport]] |
| - | [[Category: | + | [[Category: Transport]] |
| - | [[Category: | + | [[Category: Vps36]] |
| - | [[Category: | + | [[Category: Zinc]] |
| - | [[Category: | + | [[Category: Zinc-finger]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:36:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:36, 3 May 2008
VPS36 N-TERMINAL PH DOMAIN
Overview
ESCRT complexes form the main machinery driving protein sorting from endosomes to lysosomes. Currently, the picture regarding assembly of ESCRTs on endosomes is incomplete. The structure of the conserved heterotrimeric ESCRT-I core presented here shows a fan-like arrangement of three helical hairpins, each corresponding to a different subunit. Vps23/Tsg101 is the central hairpin sandwiched between the other subunits, explaining the critical role of its "steadiness box" in the stability of ESCRT-I. We show that yeast ESCRT-I links directly to ESCRT-II, through a tight interaction of Vps28 (ESCRT-I) with the yeast-specific zinc-finger insertion within the GLUE domain of Vps36 (ESCRT-II). The crystal structure of the GLUE domain missing this insertion reveals it is a split PH domain, with a noncanonical lipid binding pocket that binds PtdIns3P. The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes.
About this Structure
2CAY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes., Teo H, Gill DJ, Sun J, Perisic O, Veprintsev DB, Vallis Y, Emr SD, Williams RL, Cell. 2006 Apr 7;125(1):99-111. PMID:16615893 Page seeded by OCA on Sat May 3 21:36:45 2008
