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2ccz
From Proteopedia
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[[Image:2ccz.gif|left|200px]] | [[Image:2ccz.gif|left|200px]] | ||
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'''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA''' | '''CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA''' | ||
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[[Category: Sun, Y J.]] | [[Category: Sun, Y J.]] | ||
[[Category: Wu, H N.]] | [[Category: Wu, H N.]] | ||
| - | [[Category: | + | [[Category: Primosome,prib,dna replication,dna repair,dna recombination,ssdna,single-stranded dna,dna/protein complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:50:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 18:50, 3 May 2008
CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA
Overview
PriB is a primosomal protein required for replication restart in Escherichia coli. PriB stimulates PriA helicase activity via interaction with single-stranded DNA (ssDNA), but the molecular details of this interaction remain unclear. Here, we report the crystal structure of PriB complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB shares structural similarity with the E.coli ssDNA-binding protein (EcoSSB). However, the structure of the PriB-dT15 complex reveals that PriB binds ssDNA differently. Results from filter-binding assays show that PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational analysis suggests that the loop L45 plays an important role in ssDNA binding. Based on the crystal structure and biochemical analyses, we propose a cooperative mechanism for the binding of PriB to ssDNA and a model for the assembly of the PriA-PriB-ssDNA complex. This report presents the first structure of a replication restart primosomal protein complexed with DNA, and a novel model that explains the interactions between a dimeric oligonucleotide-binding-fold protein and ssDNA.
About this Structure
2CCZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode., Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD, Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:16899446 Page seeded by OCA on Sat May 3 21:50:45 2008
