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2cfa

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[[Image:2cfa.gif|left|200px]]
[[Image:2cfa.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2cfa |SIZE=350|CAPTION= <scene name='initialview01'>2cfa</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2cfa", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Fad+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2cfa| PDB=2cfa | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB]</span>
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}}
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'''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX'''
'''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX'''
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[[Category: Paramecium bursaria chlorella virus 1]]
[[Category: Paramecium bursaria chlorella virus 1]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Thymidylate synthase (FAD)]]
 
[[Category: Bernauer, J.]]
[[Category: Bernauer, J.]]
[[Category: Decottignies, P.]]
[[Category: Decottignies, P.]]
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[[Category: Tilbeurgh, H Van.]]
[[Category: Tilbeurgh, H Van.]]
[[Category: Zhou, C Z.]]
[[Category: Zhou, C Z.]]
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[[Category: fdt]]
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[[Category: Fdt]]
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[[Category: flavin dependent thymidylate synthase fad]]
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[[Category: Flavin dependent thymidylate synthase fad]]
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[[Category: flavoprotein]]
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[[Category: Flavoprotein]]
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[[Category: methyltransferase]]
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[[Category: Methyltransferase]]
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[[Category: nucleotide biosynthesis]]
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[[Category: Nucleotide biosynthesis]]
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[[Category: paramecium bursaria chlorella virus-1]]
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[[Category: Paramecium bursaria chlorella virus-1]]
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[[Category: thyx]]
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[[Category: Thyx]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: tscp]]
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[[Category: Tscp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:59:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:20:39 2008''
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Revision as of 18:59, 3 May 2008

Image:2cfa.gif

Template:STRUCTURE 2cfa

STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX


Overview

By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.

About this Structure

2CFA is a Protein complex structure of sequences from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.

Reference

Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:16707489 Page seeded by OCA on Sat May 3 21:59:40 2008

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