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2cme

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cme OCA], [http://www.ebi.ac.uk/pdbsum/2cme PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cme RCSB]</span>
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'''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN'''
'''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN'''
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[[Category: Meier, C.]]
[[Category: Meier, C.]]
[[Category: Stuart, D I.]]
[[Category: Stuart, D I.]]
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[[Category: alternative open reading frame]]
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[[Category: Alternative open reading frame]]
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[[Category: hypothetical protein]]
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[[Category: Hypothetical protein]]
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[[Category: lipid-binding]]
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[[Category: Lipid-binding]]
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[[Category: virus assembly]]
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[[Category: Virus assembly]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:30:25 2008''
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Revision as of 19:30, 3 May 2008

Template:STRUCTURE 2cme

THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN


Overview

To achieve the greatest output from their limited genomes, viruses frequently make use of alternative open reading frames, in which translation is initiated from a start codon within an existing gene and, being out of frame, gives rise to a distinct protein product. These alternative protein products are, as yet, poorly characterized structurally. Here we report the crystal structure of ORF-9b, an alternative open reading frame within the nucleocapsid (N) gene from the SARS coronavirus. The protein has a novel fold, a dimeric tent-like beta structure with an amphipathic surface, and a central hydrophobic cavity that binds lipid molecules. This cavity is likely to be involved in membrane attachment and, in mammalian cells, ORF-9b associates with intracellular vesicles, consistent with a role in the assembly of the virion. Analysis of ORF-9b and other overlapping genes suggests that they provide snapshots of the early evolution of novel protein folds.

About this Structure

2CME is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.

Reference

The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus., Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RJ, Grimes JM, Stuart DI, Structure. 2006 Jul;14(7):1157-65. PMID:16843897 Page seeded by OCA on Sat May 3 22:30:25 2008

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