2d3i
From Proteopedia
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'''Crystal Structure of Aluminum-Bound Ovotransferrin at 2.15 Angstrom Resolution''' | '''Crystal Structure of Aluminum-Bound Ovotransferrin at 2.15 Angstrom Resolution''' | ||
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[[Category: Mikami, B.]] | [[Category: Mikami, B.]] | ||
[[Category: Mizutani, K.]] | [[Category: Mizutani, K.]] | ||
| - | [[Category: | + | [[Category: Aluminum]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | [[Category: | + | [[Category: Transferrin]] |
| - | [[Category: | + | [[Category: Transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:38:06 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:38, 3 May 2008
Crystal Structure of Aluminum-Bound Ovotransferrin at 2.15 Angstrom Resolution
Overview
Transferrin, well known as an iron-transport protein, can bind other metal ions, including toxic ones, and is considered to play an important role in the transportation of such metal ions. Here, a crystal structure of aluminium-bound transferrin is described for the first time. Colourless needle-shaped crystals of aluminium-bound ovotransferrin were obtained in PEG 400 solution. Structural determination was performed by molecular replacement using diferric (iron-bound) ovotransferrin as a model and the structural refinement was performed in the 50-2.15 Angstroms resolution range. The overall organization of the aluminium-bound form is almost the same as the iron-bound form: the protein is folded into two homologous lobes (N- and C-lobes) with two domains; two metal-binding sites are located within the inter-domain clefts of each lobe. Four residues (one Asp, two Tyr and one His) and one bicarbonate anion were found to bind an aluminium ion in either lobe, as in the iron-bound form. The highly similar domain-closed structure of the Al(3+)-bound form may permit the binding of Al(3+)-bound transferrin to the transferrin receptor. An unusual interaction, the dilysine trigger, which facilitates iron release at low pH in the endosome, was also found in the Al(3+)-bound form. These findings support the participation of transferrin in the transport of Al(3+) ions in vivo.
About this Structure
2D3I is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Structure of aluminium-bound ovotransferrin at 2.15 Angstroms resolution., Mizutani K, Mikami B, Aibara S, Hirose M, Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1636-42. Epub 2005, Nov 19. PMID:16301797 Page seeded by OCA on Sat May 3 23:38:06 2008
