2dci
From Proteopedia
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[[Image:2dci.gif|left|200px]] | [[Image:2dci.gif|left|200px]] | ||
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'''NMR structure of influenza HA fusion peptide mutant W14A in DPC in pH5''' | '''NMR structure of influenza HA fusion peptide mutant W14A in DPC in pH5''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DCI is a [[Single protein]] structure | + | 2DCI is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DCI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lai, A L.]] | [[Category: Lai, A L.]] | ||
[[Category: Tamm, L K.]] | [[Category: Tamm, L K.]] | ||
| - | [[Category: | + | [[Category: Fusion peptide]] |
| - | [[Category: | + | [[Category: Ha]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:10:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:10, 3 May 2008
NMR structure of influenza HA fusion peptide mutant W14A in DPC in pH5
Overview
The fusion peptide of influenza hemagglutinin is crucial for cell entry of this virus. Previous studies showed that this peptide adopts a boomerang-shaped structure in lipid model membranes at the pH of membrane fusion. To examine the role of the boomerang in fusion, we changed several residues proposed to stabilize the kink in this structure and measured fusion. Among these, mutants E11A and W14A expressed hemagglutinins with hemifusion and no fusion activities, and F9A and N12A had no effect on fusion, respectively. Binding enthalpies and free energies of mutant peptides to model membranes and their ability to perturb lipid bilayer structures correlated well with the fusion activities of the parent full-length molecules. The structure of W14A determined by NMR and site-directed spin labeling features a flexible kink that points out of the membrane, in sharp contrast to the more ordered boomerang of the wild-type, which points into the membrane. A specific fixed angle boomerang structure is thus required to support membrane fusion.
About this Structure
2DCI is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Fusion peptide of influenza hemagglutinin requires a fixed angle boomerang structure for activity., Lai AL, Park H, White JM, Tamm LK, J Biol Chem. 2006 Mar 3;281(9):5760-70. Epub 2005 Dec 28. PMID:16407195 Page seeded by OCA on Sun May 4 00:10:14 2008
