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2dep
From Proteopedia
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'''Crystal Structure of xylanase B from Clostridium stercorarium F9''' | '''Crystal Structure of xylanase B from Clostridium stercorarium F9''' | ||
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[[Category: Miyanaga, A.]] | [[Category: Miyanaga, A.]] | ||
[[Category: Nishimoto, M.]] | [[Category: Nishimoto, M.]] | ||
| - | [[Category: | + | [[Category: Family 10]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: National project on protein structural and functional analyse]] |
| - | [[Category: | + | [[Category: Nppsfa]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Xylan degradation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:17:07 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 21:17, 3 May 2008
Crystal Structure of xylanase B from Clostridium stercorarium F9
Overview
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
About this Structure
2DEP is a Single protein structure of sequence from Clostridium stercorarium. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719 Page seeded by OCA on Sun May 4 00:17:07 2008
