2dep

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[[Image:2dep.jpg|left|200px]]
[[Image:2dep.jpg|left|200px]]
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{{Structure
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|GENE= xynB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1510 Clostridium stercorarium])
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{{STRUCTURE_2dep| PDB=2dep | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dep FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dep OCA], [http://www.ebi.ac.uk/pdbsum/2dep PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dep RCSB]</span>
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'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''
'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''
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[[Category: Miyanaga, A.]]
[[Category: Miyanaga, A.]]
[[Category: Nishimoto, M.]]
[[Category: Nishimoto, M.]]
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[[Category: family 10]]
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[[Category: Family 10]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: nppsfa]]
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[[Category: Nppsfa]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: xylan degradation]]
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[[Category: Xylan degradation]]
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Revision as of 21:17, 3 May 2008

Template:STRUCTURE 2dep

Crystal Structure of xylanase B from Clostridium stercorarium F9


Overview

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.

About this Structure

2DEP is a Single protein structure of sequence from Clostridium stercorarium. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719 Page seeded by OCA on Sun May 4 00:17:07 2008

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