2df4

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[[Image:2df4.gif|left|200px]]
[[Image:2df4.gif|left|200px]]
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{{Structure
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|PDB= 2df4 |SIZE=350|CAPTION= <scene name='initialview01'>2df4</scene>, resolution 3.2&Aring;
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The line below this paragraph, containing "STRUCTURE_2df4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= gatA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]), gatB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]), gatC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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|DOMAIN=
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{{STRUCTURE_2df4| PDB=2df4 | SCENE= }}
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|RELATEDENTRY=[[2f2a|2F2A]], [[2g5h|2G5H]], [[2g5i|2G5I]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2df4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2df4 OCA], [http://www.ebi.ac.uk/pdbsum/2df4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2df4 RCSB]</span>
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}}
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'''Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Mn2+'''
'''Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Mn2+'''
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[[Category: Tanaka, I.]]
[[Category: Tanaka, I.]]
[[Category: Yao, M.]]
[[Category: Yao, M.]]
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[[Category: multi protein complex]]
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[[Category: Multi protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:18:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:33:52 2008''
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Revision as of 21:18, 3 May 2008

Image:2df4.gif

Template:STRUCTURE 2df4

Structure of tRNA-Dependent Amidotransferase GatCAB complexed with Mn2+


Overview

The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 A in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gln-tRNA(Gln).

About this Structure

2DF4 is a Protein complex structure of sequences from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Ammonia channel couples glutaminase with transamidase reactions in GatCAB., Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I, Science. 2006 Jun 30;312(5782):1954-8. PMID:16809541 Page seeded by OCA on Sun May 4 00:18:21 2008

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