2dhc

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[[Image:2dhc.jpg|left|200px]]
[[Image:2dhc.jpg|left|200px]]
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{{Structure
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|PDB= 2dhc |SIZE=350|CAPTION= <scene name='initialview01'>2dhc</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_2dhc", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=DCE:1,2-DICHLOROETHANE'>DCE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span>
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|GENE=
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{{STRUCTURE_2dhc| PDB=2dhc | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dhc OCA], [http://www.ebi.ac.uk/pdbsum/2dhc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dhc RCSB]</span>
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}}
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'''CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE'''
'''CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE'''
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[[Category: Dijkstra, B W.]]
[[Category: Dijkstra, B W.]]
[[Category: Verschueren, K H.G.]]
[[Category: Verschueren, K H.G.]]
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[[Category: dehalogenase]]
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[[Category: Dehalogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:25:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:34:43 2008''
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Revision as of 21:25, 3 May 2008

Image:2dhc.jpg

Template:STRUCTURE 2dhc

CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE


Overview

Crystal structures of haloalkane dehalogenase were determined in the presence of the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in the active site without being converted; warming to room temperature causes the substrate's carbon-chlorine bond to be broken, producing a chloride ion with concomitant alkylation of the active-site residue Asp124. At pH 6 and room temperature the alkylated enzyme is hydrolysed by a water molecule activated by the His289-Asp260 pair in the active site. These results show that catalysis by the dehalogenase proceeds by a two-step mechanism involving an ester intermediate covalently bound at Asp124.

About this Structure

2DHC is a Single protein structure of sequence from Xanthobacter autotrophicus. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase., Verschueren KH, Seljee F, Rozeboom HJ, Kalk KH, Dijkstra BW, Nature. 1993 Jun 24;363(6431):693-8. PMID:8515812 Page seeded by OCA on Sun May 4 00:25:50 2008

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