2di2
From Proteopedia
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[[Image:2di2.jpg|left|200px]] | [[Image:2di2.jpg|left|200px]] | ||
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'''NMR structure of the HIV-2 nucleocapsid protein''' | '''NMR structure of the HIV-2 nucleocapsid protein''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DI2 is a [[Single protein]] structure | + | 2DI2 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DI2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sato, K.]] | [[Category: Sato, K.]] | ||
[[Category: Tanaka, T.]] | [[Category: Tanaka, T.]] | ||
| - | [[Category: | + | [[Category: Hiv-2]] |
| - | [[Category: | + | [[Category: Mutant]] |
| - | [[Category: | + | [[Category: Nucleocapsid protein]] |
| - | [[Category: | + | [[Category: Rna recognition]] |
| - | [[Category: | + | [[Category: Zinc finger]] |
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Revision as of 21:27, 3 May 2008
NMR structure of the HIV-2 nucleocapsid protein
Overview
NCp8 of HIV-2 contains two CCHC-type zinc fingers connected by a linker, and is involved in many critical steps of the virus life cycle. It was previously shown that the first zinc finger flanked by the linker is the minimal active domain for specific binding to viral RNA. In our previous study, we determined the three-dimensional structure of NCp8-f1, including the minimal active domain, and found that a hydrogen bond between Asn(11) N(delta)H and Arg(27) O stabilized the conformation of the linker in the vicinity of the zinc finger [Kodera et al. (1998) Biochemistry 37, 17704-17713]. In this study, RNA binding activities of NCp8-f1 and three types of its mutant peptides were analysed by native PAGE assay. The activity and three-dimensional structure of NCp8-f1/N11A, in which alanine is substituted for Asn(11) thereby affecting the conformation of the linker, was analyzed and compared with those of NCp8-f1. We demonstrated that the existence of Arg(4) and/or Lys(5) and Arg(26) and/or Arg(27) were necessary for binding RNA. Furthermore, the linker's flexible orientation, which is controlled by the hydrogen bond between Asn(11) N(delta)H and Arg(27) O, appears to be a structural basis for NCp8 existing as a multi-functional protein.
About this Structure
2DI2 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
RNA recognition mechanism of the minimal active domain of the human immunodeficiency virus type-2 nucleocapsid protein., Matsui T, Kodera Y, Endoh H, Miyauchi E, Komatsu H, Sato K, Tanaka T, Kohno T, Maeda T, J Biochem. 2007 Feb;141(2):269-77. Epub 2007 Jan 3. PMID:17202191 Page seeded by OCA on Sun May 4 00:27:48 2008
Categories: Single protein | Endoh, H. | Kodera, Y. | Kohno, T. | Komatsu, H. | Maeda, T. | Matsui, T. | Miyauchi, E. | Sato, K. | Tanaka, T. | Hiv-2 | Mutant | Nucleocapsid protein | Rna recognition | Zinc finger
