2dpt

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[[Image:2dpt.jpg|left|200px]]
[[Image:2dpt.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2dpt |SIZE=350|CAPTION= <scene name='initialview01'>2dpt</scene>, resolution 2.75&Aring;
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The line below this paragraph, containing "STRUCTURE_2dpt", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PUY:PUROMYCIN'>PUY</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucyltransferase Leucyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.6 2.3.2.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2dpt| PDB=2dpt | SCENE= }}
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|RELATEDENTRY=[[2dps|2DPS]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpt OCA], [http://www.ebi.ac.uk/pdbsum/2dpt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dpt RCSB]</span>
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}}
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'''Leucyl/phenylalanyl-tRNA-protein transferase complexed with puromycin'''
'''Leucyl/phenylalanyl-tRNA-protein transferase complexed with puromycin'''
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[[Category: Suto, K.]]
[[Category: Suto, K.]]
[[Category: Tomita, K.]]
[[Category: Tomita, K.]]
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[[Category: aminoacyl-trna-protein transferase]]
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[[Category: Aminoacyl-trna-protein transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:55:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:37:49 2008''
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Revision as of 21:56, 3 May 2008

Image:2dpt.jpg

Template:STRUCTURE 2dpt

Leucyl/phenylalanyl-tRNA-protein transferase complexed with puromycin


Overview

Eubacterial leucyl/phenylalanyl-tRNA protein transferase (L/F-transferase), encoded by the aat gene, conjugates leucine or phenylalanine to the N-terminal Arg or Lys residue of proteins, using Leu-tRNA(Leu) or Phe-tRNA(Phe) as a substrate. The resulting N-terminal Leu or Phe acts as a degradation signal for the ClpS-ClpAP-mediated N-end rule protein degradation pathway. Here, we present the crystal structures of Escherichia coli L/F-transferase and its complex with an aminoacyl-tRNA analog, puromycin. The C-terminal domain of L/F-transferase consists of the GCN5-related N-acetyltransferase fold, commonly observed in the acetyltransferase superfamily. The p-methoxybenzyl group of puromycin, corresponding to the side chain of Leu or Phe of Leu-tRNA(Leu) or Phe-tRNA(Phe), is accommodated in a highly hydrophobic pocket, with a shape and size suitable for hydrophobic amino-acid residues lacking a branched beta-carbon, such as leucine and phenylalanine. Structure-based mutagenesis of L/F-transferase revealed its substrate specificity. Furthermore, we present a model of the L/F-transferase complex with tRNA and substrate proteins bearing an N-terminal Arg or Lys.

About this Structure

2DPT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of leucyl/phenylalanyl-tRNA-protein transferase and its complex with an aminoacyl-tRNA analog., Suto K, Shimizu Y, Watanabe K, Ueda T, Fukai S, Nureki O, Tomita K, EMBO J. 2006 Dec 13;25(24):5942-50. Epub 2006 Nov 16. PMID:17110926 Page seeded by OCA on Sun May 4 00:55:59 2008

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