2dr2

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[[Image:2dr2.gif|left|200px]]
[[Image:2dr2.gif|left|200px]]
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{{Structure
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|PDB= 2dr2 |SIZE=350|CAPTION= <scene name='initialview01'>2dr2</scene>, resolution 3.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2dr2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= WARS, WRS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2dr2| PDB=2dr2 | SCENE= }}
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|RELATEDENTRY=[[1o5t|1O5T]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dr2 OCA], [http://www.ebi.ac.uk/pdbsum/2dr2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dr2 RCSB]</span>
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}}
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'''Structure of human tryptophanyl-tRNA synthetase in complex with tRNA(Trp)'''
'''Structure of human tryptophanyl-tRNA synthetase in complex with tRNA(Trp)'''
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[[Category: Shen, N.]]
[[Category: Shen, N.]]
[[Category: Yang, B.]]
[[Category: Yang, B.]]
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[[Category: rossmann fold]]
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[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:59:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:16 2008''
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Revision as of 21:59, 3 May 2008

Template:STRUCTURE 2dr2

Structure of human tryptophanyl-tRNA synthetase in complex with tRNA(Trp)


Overview

Aminoacyl-tRNA synthetases (aaRSs) are a family of enzymes responsible for the covalent link of amino acids to their cognate tRNAs. The selectivity and species-specificity in the recognitions of both amino acid and tRNA by aaRSs play a vital role in maintaining the fidelity of protein synthesis. We report here the first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. hTrpRS recognizes the tRNA acceptor arm from the major groove; however, the 3' end CCA of the tRNA makes a sharp turn to bind at the active site with a deformed conformation. The discriminator base A73 is specifically recognized by an alpha-helix of the unique N-terminal domain and the anticodon loop by an alpha-helix insertion of the C-terminal domain. The N-terminal domain appears to be involved in Trp activation, but not essential for tRNA binding and acylation. Structural and sequence comparisons suggest that this novel tRNA binding and recognition mechanism is very likely shared by other archaeal and eukaryotic TrpRSs, but not by bacterial TrpRSs. Our findings provide insights into the molecular basis of tRNA specificity and species-specificity.

About this Structure

2DR2 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity., Shen N, Guo L, Yang B, Jin Y, Ding J, Nucleic Acids Res. 2006 Jun 23;34(11):3246-58. Print 2006. PMID:16798914 Page seeded by OCA on Sun May 4 00:59:34 2008

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