2ds0

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[[Image:2ds0.jpg|left|200px]]
[[Image:2ds0.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2ds0 |SIZE=350|CAPTION= <scene name='initialview01'>2ds0</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_2ds0", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+B+270'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+271'>AC2</scene>, <scene name='pdbsite=AC3:Sia+Binding+Site+For+Residue+A+280'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+281'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+1001'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+1002'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+1003'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+A+1004'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+B+1005'>AC9</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2ds0| PDB=2ds0 | SCENE= }}
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|RELATEDENTRY=[[2ao3|2AO3]], [[2d12|2D12]], [[2dry|2DRY]], [[2drz|2DRZ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ds0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ds0 OCA], [http://www.ebi.ac.uk/pdbsum/2ds0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ds0 RCSB]</span>
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}}
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'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''
'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''
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[[Category: Fujimoto, Z.]]
[[Category: Fujimoto, Z.]]
[[Category: Suzuki, R.]]
[[Category: Suzuki, R.]]
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[[Category: beta-trefoil fold]]
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[[Category: Beta-trefoil fold]]
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[[Category: earthworm lumbricus terrestri]]
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[[Category: Earthworm lumbricus terrestri]]
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[[Category: galactose]]
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[[Category: Galactose]]
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[[Category: in vitro evolution]]
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[[Category: In vitro evolution]]
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[[Category: sialic acid]]
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[[Category: Sialic acid]]
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[[Category: sugar binding protein]]
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[[Category: Sugar binding protein]]
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[[Category: sugar complex]]
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[[Category: Sugar complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:04:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:40 2008''
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Revision as of 22:04, 3 May 2008

Image:2ds0.jpg

Template:STRUCTURE 2ds0

Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose


Overview

Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.

About this Structure

2DS0 is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.

Reference

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683 Page seeded by OCA on Sun May 4 01:04:33 2008

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