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2ds3

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[[Image:2ds3.gif|left|200px]]
[[Image:2ds3.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ds3 |SIZE=350|CAPTION= <scene name='initialview01'>2ds3</scene>, resolution 1.78&Aring;
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The line below this paragraph, containing "STRUCTURE_2ds3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= ygjK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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|DOMAIN=
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{{STRUCTURE_2ds3| PDB=2ds3 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ds3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ds3 OCA], [http://www.ebi.ac.uk/pdbsum/2ds3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ds3 RCSB]</span>
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}}
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'''Crystal structure of E. coli YgjK, a member of glycosyl hydrolase family 63'''
'''Crystal structure of E. coli YgjK, a member of glycosyl hydrolase family 63'''
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[[Category: Kurakata, Y.]]
[[Category: Kurakata, Y.]]
[[Category: Tonozuka, T.]]
[[Category: Tonozuka, T.]]
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[[Category: escherichia coli]]
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[[Category: Escherichia coli]]
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[[Category: glycosyl hydrolase family 63]]
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[[Category: Glycosyl hydrolase family 63]]
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[[Category: processing alpha-glucosidase]]
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[[Category: Processing alpha-glucosidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:05:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:42 2008''
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Revision as of 22:05, 3 May 2008

Image:2ds3.gif

Template:STRUCTURE 2ds3

Crystal structure of E. coli YgjK, a member of glycosyl hydrolase family 63


Overview

Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.

About this Structure

2DS3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:15213393 Page seeded by OCA on Sun May 4 01:05:19 2008

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