2e4i
From Proteopedia
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[[Image:2e4i.gif|left|200px]] | [[Image:2e4i.gif|left|200px]] | ||
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'''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines''' | '''Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2E4I is a [[Single protein]] structure | + | 2E4I is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E4I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sugiyama, H.]] | [[Category: Sugiyama, H.]] | ||
[[Category: Xu, Y.]] | [[Category: Xu, Y.]] | ||
| - | [[Category: | + | [[Category: Dna]] |
| - | [[Category: | + | [[Category: Mixed-parallel/antiparallel]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Quadruplex]] |
| - | [[Category: | + | [[Category: Structure]] |
| - | [[Category: | + | [[Category: Telomere]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:54:11 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 22:54, 3 May 2008
Human Telomeric DNA mixed-parallel/antiparallel quadruplex under Physiological Ionic Conditions Stabilized by Proper Incorporation of 8-Bromoguanosines
Overview
The structure of human telomeric DNA is controversial; it depends upon the sequence contexts and the methodologies used to determine it. The solution structure in the presence of K(+) is particularly interesting, but the structure is yet to be elucidated, due to possible conformational heterogeneity. Here, a unique strategy is applied to stabilize one such structure in a K(+) solution by substituting guanosines with 8-bromoguanosines at proper positions. The resulting spectra are cleaner and led to determination of the structure at a high atomic resolution. This demonstrates that the application of 8-bromoguanosine is a powerful tool to overcome the difficulty of nucleic acid structure determination arising from conformational heterogeneity. The obtained structure is a mixed-parallel/antiparallel quadruplex. The structure of telomeric DNA was recently reported in another study, in which stabilization was brought about by mutation and resultant additional interactions [Luu KN, Phan AT, Kuryavyi V, Lacroix L & Patel DJ (2006) Structure of the human telomere in K(+) solution: an intramolecular (3+1) G-quadruplex scaffold. J Am Chem Soc 128, 9963-9970]. The structure of the guanine tracts was similar between the two. However, a difference was seen for loops connecting guanine tracts, which may play a role in the higher order arrangement of telomeres. Our structure can be utilized to design a small molecule which stabilizes the quadruplex. This type of molecule is supposed to inhibit a telomerase and thus is expected to be a candidate anticancer drug.
About this Structure
2E4I is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Structure of a human telomeric DNA sequence stabilized by 8-bromoguanosine substitutions, as determined by NMR in a K+ solution., Matsugami A, Xu Y, Noguchi Y, Sugiyama H, Katahira M, FEBS J. 2007 Jul;274(14):3545-56. Epub 2007 Jun 11. PMID:17561958 Page seeded by OCA on Sun May 4 01:54:11 2008
Categories: Single protein | Katahira, M. | Matsugami, A. | Noguchi, Y. | Sugiyama, H. | Xu, Y. | Dna | Mixed-parallel/antiparallel | Nmr | Quadruplex | Structure | Telomere
