2e7p
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2e7p.jpg|left|200px]] | [[Image:2e7p.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2e7p", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_2e7p| PDB=2e7p | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides''' | '''Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides''' | ||
| Line 33: | Line 30: | ||
[[Category: Takahashi, T.]] | [[Category: Takahashi, T.]] | ||
[[Category: Unno, H.]] | [[Category: Unno, H.]] | ||
| - | [[Category: | + | [[Category: Electron transport]] |
| - | [[Category: | + | [[Category: Glutaredoxin]] |
| - | [[Category: | + | [[Category: Poplar]] |
| - | [[Category: | + | [[Category: Thioredoxin fold]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:05:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:05, 3 May 2008
Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides
Overview
When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.
About this Structure
2E7P is a Single protein structure of sequence from Populus tremula x populus tremuloides. Full crystallographic information is available from OCA.
Reference
Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1., Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP, Proc Natl Acad Sci U S A. 2007 May 1;104(18):7379-84. Epub 2007 Apr 25. PMID:17460036 Page seeded by OCA on Sun May 4 02:05:22 2008
