2ec0
From Proteopedia
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'''RNA-dependent RNA polymerase of foot-and-mouth disease virus in complex with a template-primer RNA and ATP''' | '''RNA-dependent RNA polymerase of foot-and-mouth disease virus in complex with a template-primer RNA and ATP''' | ||
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[[Category: Verdaguer, N.]] | [[Category: Verdaguer, N.]] | ||
[[Category: 3d polymerase]] | [[Category: 3d polymerase]] | ||
| - | [[Category: | + | [[Category: Foot-and- mouth disease virus]] |
| - | [[Category: | + | [[Category: Polymerase]] |
| - | [[Category: | + | [[Category: Rna-dependent rna polymerase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:18:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 23:18, 3 May 2008
RNA-dependent RNA polymerase of foot-and-mouth disease virus in complex with a template-primer RNA and ATP
Overview
RNA virus replication is an error-prone event caused by the low fidelity of viral RNA-dependent RNA polymerases. Replication fidelity can be decreased further by the use of mutagenic ribonucleoside analogs to a point where viral genetic information can no longer be maintained. For foot-and-mouth disease virus, the antiviral analogs ribavirin and 5-fluorouracil have been shown to be mutagenic, contributing to virus extinction through lethal mutagenesis. Here, we report the x-ray structure of four elongation complexes of foot-and-mouth disease virus polymerase 3D obtained in presence of natural substrates, ATP and UTP, or mutagenic nucleotides, ribavirin triphosphate and 5-fluorouridine triphosphate with different RNAs as template-primer molecules. The ability of these complexes to synthesize RNA in crystals allowed us to capture different successive replication events and to define the critical amino acids involved in (i) the recognition and positioning of the incoming nucleotide or analog; (ii) the positioning of the acceptor base of the template strand; and (iii) the positioning of the 3'-OH group of the primer nucleotide during RNA replication. The structures identify key interactions involved in viral RNA replication and provide insights into the molecular basis of the low fidelity of viral RNA polymerases.
About this Structure
2EC0 is a Single protein structure of sequence from Foot-and-mouth disease virus. Full crystallographic information is available from OCA.
Reference
Sequential structures provide insights into the fidelity of RNA replication., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9463-8. Epub 2007 May 21. PMID:17517631 Page seeded by OCA on Sun May 4 02:18:29 2008
