2eff

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[[Image:2eff.jpg|left|200px]]
[[Image:2eff.jpg|left|200px]]
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{{Structure
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|PDB= 2eff |SIZE=350|CAPTION= <scene name='initialview01'>2eff</scene>, resolution 1.8&Aring;
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The line below this paragraph, containing "STRUCTURE_2eff", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>
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|GENE= CYAY, B3807 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_2eff| PDB=2eff | SCENE= }}
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|RELATEDENTRY=[[1ew4|1EW4]], [[1soy|1SOY]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eff OCA], [http://www.ebi.ac.uk/pdbsum/2eff PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eff RCSB]</span>
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'''Crystal structure analysis of the complex between CyaY and Co(II)'''
'''Crystal structure analysis of the complex between CyaY and Co(II)'''
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[[Category: Franzese, M.]]
[[Category: Franzese, M.]]
[[Category: Sica, F.]]
[[Category: Sica, F.]]
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[[Category: frataxin]]
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[[Category: Frataxin]]
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[[Category: friedreich's ataxia iron binding]]
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[[Category: Friedreich's ataxia iron binding]]
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[[Category: iron-sulfur cluster assembly,detoxifying redox-active iron]]
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[[Category: Iron-sulfur cluster assembly,detoxifying redox-active iron]]
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[[Category: unknown function]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:28:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:48:14 2008''
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Revision as of 23:28, 3 May 2008

Image:2eff.jpg

Template:STRUCTURE 2eff

Crystal structure analysis of the complex between CyaY and Co(II)


Overview

Deficiency of the small mitochondrial protein frataxin causes Friedreich's ataxia, a severe neurodegenerative pathology. Frataxin, which has been highly conserved throughout evolution, is thought to be involved in, among other processes, Fe-S cluster formation. Independent evidence shows that it binds iron directly, although with very distinct features and low affinity. Here, we have carried out an extensive study of the binding properties of CyaY, the bacterial ortholog of frataxin, to different divalent and trivalent cations, using NMR and X-ray crystallography. We demonstrate that the protein has low cation specificity and contains multiple binding sites able to chelate divalent and trivalent metals with low affinity. Binding does not involve cavities or pockets, but exposed glutamates and aspartates, which are residues that are unusual for iron chelation when not assisted by histidines and/or cysteines. We have related how such an ability to bind cations on a relatively large area through an electrostatic mechanism could be a valuable asset for protein function.

About this Structure

2EFF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Understanding the binding properties of an unusual metal-binding protein--a study of bacterial frataxin., Pastore C, Franzese M, Sica F, Temussi P, Pastore A, FEBS J. 2007 Aug;274(16):4199-210. Epub 2007 Jul 25. PMID:17651435 Page seeded by OCA on Sun May 4 02:28:11 2008

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