2eu8

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[[Image:2eu8.gif|left|200px]]
[[Image:2eu8.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2eu8 |SIZE=350|CAPTION= <scene name='initialview01'>2eu8</scene>, resolution 1.800&Aring;
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The line below this paragraph, containing "STRUCTURE_2eu8", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AP5:BIS(ADENOSINE)-5&#39;-PENTAPHOSPHATE'>AP5</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= adk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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-->
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|DOMAIN=
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{{STRUCTURE_2eu8| PDB=2eu8 | SCENE= }}
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|RELATEDENTRY=[[1zin|1zin]], [[1p3j|1p3j]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eu8 OCA], [http://www.ebi.ac.uk/pdbsum/2eu8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eu8 RCSB]</span>
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}}
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'''Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)'''
'''Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)'''
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[[Category: Chen, S.]]
[[Category: Chen, S.]]
[[Category: Shamoo, Y.]]
[[Category: Shamoo, Y.]]
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[[Category: adenylate kinase]]
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[[Category: Adenylate kinase]]
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[[Category: in vivo evolution]]
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[[Category: In vivo evolution]]
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[[Category: point mutant]]
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[[Category: Point mutant]]
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[[Category: thermostability]]
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[[Category: Thermostability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:07:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:53:51 2008''
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Revision as of 00:07, 4 May 2008

Image:2eu8.gif

Template:STRUCTURE 2eu8

Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)


Overview

In nature, evolution occurs through the continuous adaptation of a population to its environment. At the molecular level, adaptive changes in protein sequence and expression impact organismal fitness and, consequently, dictate population dynamics. Here, we have used a "weak link" method to favor variations in one gene, allowing adaptation to thermostability to be studied in molecular detail as bacteria were grown continuously for approximately 1500 generations. Surprisingly, only six mutant alleles, representing less than 1% of the possible missense mutations, were observed, suggesting a highly constrained molecular landscape during protein evolution. The changes in organismal fitness were linked directly to incremental increases in enzyme stability and activity maxima and corresponded to the narrow temperature ranges where each mutant enjoyed success within the overall population. Thus, continuous evolution of a single gene permits a quantitative approach that extends from the phenotypes of the microbial populations to their underlying biophysical basis.

About this Structure

2EU8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

In vivo molecular evolution reveals biophysical origins of organismal fitness., Counago R, Chen S, Shamoo Y, Mol Cell. 2006 May 19;22(4):441-9. PMID:16713575 Page seeded by OCA on Sun May 4 03:07:08 2008

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