2euw

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[[Image:2euw.gif|left|200px]]
[[Image:2euw.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2euw |SIZE=350|CAPTION= <scene name='initialview01'>2euw</scene>, resolution 1.680&Aring;
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The line below this paragraph, containing "STRUCTURE_2euw", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= NDT80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_2euw| PDB=2euw | SCENE= }}
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|RELATEDENTRY=[[1mnn|1MNN]], [[1mn4|1MN4]], [[1m6u|1M6U]], [[1m7u|1M7U]], [[2etw|2ETW]], [[2euv|2EUV]], [[2eux|2EUX]], [[2euz|2EUZ]], [[2evf|2EVF]], [[2evg|2EVG]], [[2evh|2EVH]], [[2ehi|2EHI]], [[2ehj|2EHJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2euw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2euw OCA], [http://www.ebi.ac.uk/pdbsum/2euw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2euw RCSB]</span>
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}}
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'''Structure of a Ndt80-DNA complex (MSE mutant mA4T)'''
'''Structure of a Ndt80-DNA complex (MSE mutant mA4T)'''
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[[Category: Glover, J N.]]
[[Category: Glover, J N.]]
[[Category: Lamoureux, J S.]]
[[Category: Lamoureux, J S.]]
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[[Category: beta-barrel]]
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[[Category: Beta-barrel]]
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[[Category: ig-fold transcription factor]]
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[[Category: Ig-fold transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:08:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:54:03 2008''
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Revision as of 00:08, 4 May 2008

Image:2euw.gif

Template:STRUCTURE 2euw

Structure of a Ndt80-DNA complex (MSE mutant mA4T)


Overview

The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds a DNA consensus sequence (the middle sporulation element [MSE]) to activate gene expression after the successful completion of meiotic recombination. Here we report the X-ray crystal structures of Ndt80 bound to ten distinct MSE variants. Comparison of these structures with the structure of Ndt80 bound to a consensus MSE reveals structural principles that determine the DNA binding specificity of this transcription factor. The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are recognized by arginine side chains through a combination of hydrogen bonding and cation-pi interactions. The 3' AT-rich region is recognized via minor groove contacts that sterically exclude the N2 atom of GC base pairs. The conformation of the AT-rich region is fixed by interactions with the protein that favor recognition of poly(A)-poly(T) versus mixed AT sequences through an avoidance of major groove steric clashes at 5'-ApT-3' steps.

About this Structure

2EUW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:16531239 Page seeded by OCA on Sun May 4 03:08:32 2008

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