2ez8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2ez8.gif|left|200px]]
[[Image:2ez8.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2ez8 |SIZE=350|CAPTION= <scene name='initialview01'>2ez8</scene>, resolution 1.963&Aring;
+
The line below this paragraph, containing "STRUCTURE_2ez8", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=TDL:3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-(1-CARBOXY-1-HYDROXYETHYL)-5-(2-{[HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM'>TDL</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_oxidase Pyruvate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.3.3 1.2.3.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= pox5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1590 Lactobacillus plantarum])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2ez8| PDB=2ez8 | SCENE= }}
-
|RELATEDENTRY=[[1pow|1POW]], [[1pox|1POX]], [[1y9d|1Y9D]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ez8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ez8 OCA], [http://www.ebi.ac.uk/pdbsum/2ez8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ez8 RCSB]</span>
+
-
}}
+
'''Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate'''
'''Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate'''
Line 32: Line 29:
[[Category: Tittmann, K.]]
[[Category: Tittmann, K.]]
[[Category: Wille, G.]]
[[Category: Wille, G.]]
-
[[Category: reaction intermediate]]
+
[[Category: Reaction intermediate]]
-
[[Category: tpp enzyme]]
+
[[Category: Tpp enzyme]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:41 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:55:42 2008''
+

Revision as of 00:16, 4 May 2008

Image:2ez8.gif

Template:STRUCTURE 2ez8

Pyruvate oxidase variant F479W in complex with reaction intermediate 2-lactyl-thiamin diphosphate


Overview

Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here, we report the X-ray structures of key intermediates in the oxidative decarboxylation of pyruvate, a central reaction in carbon metabolism catalyzed by the ThDP- and flavin-dependent enzyme pyruvate oxidase (POX)3 from Lactobacillus plantarum. The structures of 2-lactyl-ThDP (LThDP, 2) and its stable phosphonate analog, of 2-hydroxyethyl-ThDP (HEThDP, 3) enamine and of 2-acetyl-ThDP (AcThDP, 4; all shown bound to the enzyme's active site) provide profound insights into the chemical mechanisms and the stereochemical course of thiamin catalysis. These snapshots also suggest a mechanism for a phosphate-linked acyl transfer coupled to electron transfer in a radical reaction of pyruvate oxidase.

About this Structure

2EZ8 is a Single protein structure of sequence from Lactobacillus plantarum. Full crystallographic information is available from OCA.

Reference

The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography., Wille G, Meyer D, Steinmetz A, Hinze E, Golbik R, Tittmann K, Nat Chem Biol. 2006 Jun;2(6):324-8. Epub 2006 May 7. PMID:16680160 Page seeded by OCA on Sun May 4 03:16:41 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ez8"
Personal tools