2f1m
From Proteopedia
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'''Conformational flexibility in the multidrug efflux system protein AcrA''' | '''Conformational flexibility in the multidrug efflux system protein AcrA''' | ||
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[[Category: Mikolosko, J.]] | [[Category: Mikolosko, J.]] | ||
[[Category: Zgurskaya, H I.]] | [[Category: Zgurskaya, H I.]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Helical hairpin]] |
| - | [[Category: | + | [[Category: Lipoyl domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:21:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:21, 4 May 2008
Conformational flexibility in the multidrug efflux system protein AcrA
Overview
Intrinsic resistance to multiple drugs in many gram-negative bacterial pathogens is conferred by resistance nodulation cell division efflux pumps, which are composed of three essential components as typified by the extensively characterized Escherichia coli AcrA-AcrB-TolC system. The inner membrane drug:proton antiporter AcrB and the outer membrane channel TolC export chemically diverse compounds out of the bacterial cell, and require the activity of the third component, the periplasmic protein AcrA. The crystal structures of AcrB and TolC have previously been determined, and we complete the molecular picture of the efflux system by presenting the structure of a stable fragment of AcrA. The AcrA fragment resembles the elongated sickle shape of its homolog Pseudomonas aeruginosa MexA, being composed of three domains: beta-barrel, lipoyl, and alpha-helical hairpin. Notably, unsuspected conformational flexibility in the alpha-helical hairpin domain of AcrA is observed, which has potential mechanistic significance in coupling between AcrA conformations and TolC channel opening.
About this Structure
2F1M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Conformational flexibility in the multidrug efflux system protein AcrA., Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P, Structure. 2006 Mar;14(3):577-87. PMID:16531241 Page seeded by OCA on Sun May 4 03:21:23 2008
