2f2h

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[[Image:2f2h.gif|left|200px]]
[[Image:2f2h.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2f2h |SIZE=350|CAPTION= <scene name='initialview01'>2f2h</scene>, resolution 1.950&Aring;
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The line below this paragraph, containing "STRUCTURE_2f2h", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XTG:4-NITROPHENYL+6-THIO-6-S-ALPHA-D-XYLOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>XTG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= yicI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2f2h| PDB=2f2h | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f2h OCA], [http://www.ebi.ac.uk/pdbsum/2f2h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f2h RCSB]</span>
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}}
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'''Structure of the YicI thiosugar Michaelis complex'''
'''Structure of the YicI thiosugar Michaelis complex'''
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[[Category: Strynadka, N C.J.]]
[[Category: Strynadka, N C.J.]]
[[Category: Withers, S G.]]
[[Category: Withers, S G.]]
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[[Category: beta8alpha8 barrel]]
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[[Category: Beta8alpha8 barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:23:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:57:00 2008''
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Revision as of 00:23, 4 May 2008

Image:2f2h.gif

Template:STRUCTURE 2f2h

Structure of the YicI thiosugar Michaelis complex


Overview

For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.

About this Structure

2F2H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex., Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG, J Am Chem Soc. 2006 Feb 22;128(7):2202-3. PMID:16478160 Page seeded by OCA on Sun May 4 03:23:13 2008

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