2f69

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[[Image:2f69.gif|left|200px]]
[[Image:2f69.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2f69 |SIZE=350|CAPTION= <scene name='initialview01'>2f69</scene>, resolution 1.30&Aring;
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The line below this paragraph, containing "STRUCTURE_2f69", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SET7, KIAA1717 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_2f69| PDB=2f69 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f69 OCA], [http://www.ebi.ac.uk/pdbsum/2f69 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f69 RCSB]</span>
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}}
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'''Ternary complex of SET7/9 bound to AdoHcy and a TAF10 peptide'''
'''Ternary complex of SET7/9 bound to AdoHcy and a TAF10 peptide'''
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[[Category: Hauk, G.]]
[[Category: Hauk, G.]]
[[Category: Trievel, R C.]]
[[Category: Trievel, R C.]]
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[[Category: enzyme-peptide-adohcy complex]]
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[[Category: Enzyme-peptide-adohcy complex]]
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[[Category: protein lysine methyltransferase]]
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[[Category: Protein lysine methyltransferase]]
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[[Category: set domain]]
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[[Category: Set domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:30:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:32 2008''
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Revision as of 00:30, 4 May 2008

Image:2f69.gif

Template:STRUCTURE 2f69

Ternary complex of SET7/9 bound to AdoHcy and a TAF10 peptide


Overview

Human SET7/9 is a protein lysine methyltransferase (PKMT) that methylates histone H3, the tumor suppressor p53 and the TBP-associated factor TAF10. To elucidate the determinants of its substrate specificity, we have solved the enzyme's structure bound to a TAF10 peptide and examined its ability to methylate histone H3, TAF10 and p53 substrates bearing either mutations or covalent modifications within their respective methylation sites. Collectively, our data reveal that SET7/9 recognizes a conserved K/R-S/T/A motif preceding the lysine substrate and has a propensity to bind aspartates and asparagines on the C-terminal side of the lysine target. We then used a sequence-based approach with this motif to identify novel substrates for this PKMT. Among the putative targets is TAF7, which is methylated at Lys5 by the enzyme in vitro. These results demonstrate the predictive value of the consensus motif in identifying novel substrates for SET7/9.

About this Structure

2F69 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for the methylation site specificity of SET7/9., Couture JF, Collazo E, Hauk G, Trievel RC, Nat Struct Mol Biol. 2006 Feb;13(2):140-6. Epub 2006 Jan 15. PMID:16415881 Page seeded by OCA on Sun May 4 03:30:34 2008

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